Fluorescent Microscopy

ISBN-13: 9781071620533 / Angielski

Bryan Heit

Opis

This volume provides both experienced and new microscopists with methods and protocols to perform fluorescence microscopy-based experiments. The book is divided into four parts detailing basic fluorescent microscopy, quantitative methods, imaging living animals, human tissue samples, approaches for imaging at a near-molecular level, and approaches to image analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Fluorescent Microscopy aims to be a useful practical guide to researches to help further their study in this field. 

Spis treści

Part I             Basic Fluorescence Imaging

 

1          Fluorescence Microscopy: A Field Guide for Biologists.

            Lucy Swift and Pina Colarusso

 

2          Three-Dimensional Simultaneous Imaging of Nucleic Acids and Proteins During Influenza Virus Infection in Single Cells Using Confocal Microscopy.

            Richard Manivanh, Seema S. Lakdawala, and Jennifer E. Jones

 

3          Optimizing Long-Term Live Cell Imaging.

            Alex Lac, Austin Le Lam, and Bryan Heit

 

Part II            Quantitative Approaches

 

4          Monitoring Transmembrane and Peripheral Membrane Protein Interactions by Förster Resonance Energy Transfer Using Fluorescence Lifetime Imaging Microscopy.

            Janhavi Nagwekar, Caterina Di Ciano-Oliveira, and Gregory D. Fairn

 

5          Bimolecular Fluorescence Complementation to Visualize Protein:Protein Interactions in Cells.

            Cassandra R. Edgar and Jimmy D. Dikeakos

 

6          Monitoring Cellular Responses to Infection with Fluorescent Biosensors.

            Amena Aktar, Kasia M Wodz, and Bryan Heit

 

7          Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF).

            Erika Jang, Siavash Ghaffari, and Warren L. Lee

 

8          Measurement of Minute Cellular Forces by Traction Force Microscopy.

            Valentin Jaumouillé

 

Part III           Tissue and Live Animal Imaging

 

9          Quantitative Immunofluorescent Imaging of Immune Cells in Mucosal Tissues.

Lane B. Buchanan, Zhongtian Shao, Yuan Chung Jiang, Abbie Lai, Thomas J. Hope, Ann M. Carias, and Jessica L. Prodger

 

10        Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin.

            Madison Turk, Jeff Biernaskie, Douglas J. Mahoney, and Craig N. Jenne

 

11        Quantifiable Intravital Light Sheet Microscopy.

Holly C. Gibbs, Sreeja Sarasamma, Oscar R. Benavides, David G. Green, Nathan A. Hart, Alvin T. Yeh, Kristen C. Maitland, and Arne C. Lekven

 

12        Hydrophobic and Hydrogel-based Methods for Passive Tissue Clearing.

Frank L. Jalufka, Sun Won Min, Madison E. Platt, Anna L. Pritchard, Theodore E. Margo, Alexandra O. Vernino, Megan A. Kirchhoff, Ryan T. Massopust, and Dylan A. McCreedy

 

13        Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.

            Ory Perelsman, Shoh Asano, and Limor Freifeld

 

Part III           Super-Resolution Approaches

 

14        Super Resolution Radial Fluctuations (SRRF) Microscopy.

            Jayme Salsman and Graham Dellaire

 

15        A Practical Guide for STED Imaging.

            Walaa Alshafie and Thomas Stroh.

 

16        Single-Molecule Localization Microscopy of Subcellular Protein Distribution in Neurons.

            Jelmer Willems, Manon Westra, and Harold D. MacGillavry

 

17        Measuring the Lateral Diffusion of Plasma Membrane Receptors using Raster Image Correlation Spectroscopy.

            Sara Makaremi and Jose Moran-Mirabal

 

18        Nanometer-Scale Molecular Mapping by Super-Resolution Fluorescence Microscopy.

            Vito Mennella and Zhen Liu

 

Part IV           Micrograph Analysis

 

19        Visualizing and Quantifying Data from Timelapse Imaging Experiments.

            Eike K. Mahlandt and Joachim Goedhart

 

20        Automated Segmentation and Microscopy Image Analysis with Machine Learning

            Anthony Bilodeau, Catherine Bouchard, and Flavie Lavoie-Cardinal