Fluorescent Methods to Study Biological Membranes

ISBN-13: 9783642331275 / Angielski / Twarda / 2012 / 486 str.

Yves M. Ly; Guy Duportail

Opis

Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Recenzje

From the reviews:

"The book covers all major applications of fluorescence to membrane studies. ... The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and cellular biologists ... . broad overview of different techniques provided by the book is also a good starting point for newcomers to the field. ... a valuable resource for everyone studying membranes and membrane proteins." (Lorenzo Stella, Analytical and Bioanalytical Chemistry, May, 2013)

Spis treści

Part I  Fluorescence Probes and Model Membranes

 

1       Laurdan Fluorescence Properties in Membranes : A Journey from the Fluorimeter to the Microscope

L.A. BAGATOLLI

 

2       Application of NBD-labeled Lipids in Membrane and Cell Biology

     S. HALDAR and A. CHATTOPADHYAY

 

3       3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: a Toolkit for Biomembrane Research

A.S. KLYMCHENKO, G. DUPORTAIL and Y. MÉLY

 

4       Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy

L.M.S. LOURA and M. PRIETO

 

5       FRET Analysis of Protein-Lipid Interactions

G. GORBENKO and P.K.J. KINNUNEN

 

6       Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shifts

Š. POKORNA, A. OLŻYŃSKA, P. JURKIEWICZ* and M. HOF

Md. JAFURULLA and A. CHATTOPADHYAY

 

Part II  Exploring Membrane Organization, Dynamics and Interactions by Advanced Fluorescence-Based Imaging Techniques

 

7       Visual Discrimination of Membrane Domains in Live Cells by Wide-Field Microscopy

C.E. BUTLER, G. WHEELER, J. GRAHAM and K.M. TYLER

 

8       Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution

D. WÜSTNER, F.W. LUND and L.M. SOLANKO

 

9       Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)

M.T. STÖCKL, R. BIZZARRI and V. SUBRAMANIAM

 

10   Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions

M. ZELMAN-FEMIAK, Y. SUBBURAJ and A.J. GARCIA-SÁEZ

 

11   Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales

V. ROUGER, C. BILLAUDEAU, T. TROMBIK, S. MAILFERT, Y. HAMON, H.-T. HE and D. MARGUET

 

12   STED-FCS Nanoscopy of Membrane Dynamics

C. EGGELING

 

13   Imaging Molecular Order in Cell Membranes by Polarization Resolved Fluorescence Microscopy

S. BRASSELET, P. FERRAND, A. KRESS, X. WANG, H. RANCHON and A. GASECKA

 

14   Near-Field Optical Nanoscopy of Biological Membranes

T.S. van ZANTEN, C. MANZO and M.F. GARCIA-PARAJO

 

Part III  Characterization of Membrane Proteins and Receptors by Advanced Fluorescence-based Imaging Techniques

  

15   Unveiling Biophysical and Biological Properties of a Hypothetical Membrane receptor by Exploiting recent Imaging Advances

P. GONNORD and R. VARMA

 

16   New Fluorescent Strategies Shine Light on the Evolving Concept of GCPR Oligomerization

M. COTTET, O. FAKLARIS, E. TRINQUET, J.-P. PIN and T. DURROUX

 

17   Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dymamics of the Serotonin_1A Receptor 

Md. JAFURULLA and A. CHATTOPADHYAY

 

18   TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy

F. NEUGART, D. WIDERA, B. KALTSCHMIDT, C. KALTSCHMIDT and M. HEILEMANN

 

19   HIV-1 Gag Directed Assembly of Retroviral Particles Investigated by Quantitative Fluorescence Imaging

H. de ROCQUIGNY, H. GACEM, P. DIDIER, J. L. DARLIX and Y. MÉLY