Fluorescence Spectroscopy and Microscopy in Biology

ISBN-13: 9783031303616 / Angielski

Radek Sachl;Mariana Amaro

Opis

This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Spis treści

Part I - Fluorescence Spectroscopy: Basics and Advanced Approaches

 

Chapter 1

Choosing the right fluorescent probe

Maria J. Sarmento (Medical University, Lisboa, Portugal) and Fábio Fernandes

 

Chapter 2

Fluorescence kinetics and time-resolved measurement

Vlastimil Fidler (Brown University, USA) and Peter Kapusta (Heyrovsky Institute, Czech Rep.)

 

Chapter 3

Quantitative Approach to Applications of Electronic Energy Transfer (EET)

Lennart B.-Å. Johansson

 

Chapter 4

Single-molecule FRET: principles and analysis

B. Israels, L. M. Lund and V. Birkedal

 

Chapter 5

Principles of fluorescence correlation and dual-color cross-correlation spectroscopy

Jan Ebenhan, Kirsten Bacia

 

Part II - Fluorescence Microscopy: Basics and Advanced Approaches

 

Chapter 6

Introduction to Fluorescence Microscopy

Radek Macháň

 

Chapter 7

Wohland STED and RESOLFT fluorescent nanoscopy

Andreas Bodén, Francesca Pennacchietti, Ilaria Testa

 

Chapter 8

Fluorescence Correlation Spectroscopy in Space and Time

Daniel Y.K. Aik, Thorsten

 

Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes

Chapter 9

Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis

Clara Bodner and Mario Brameshuber

 

Chapter 10

Quantitative photoactivated localization microscopy of membrane receptor oligomers

Marina S. Dietz and Mike Heilemann

 

Chapter 11

Diffusion measurements at the nanoscale with STED-FCS

Falk Schneider and Erdinc Sezgin

 

Chapter 12

Single molecule microscopy methods to study mitochondrial processes

Timo Dellmann, Anna Kostina, Ana J. Garcia Saez

 

Chapter 13

Transient state (TRAST) spectroscopy and imaging – exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies

Jerker Widengren

 

Chapter 14

The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET

Barbora Chmelová, Jana Humpolíčková, Kvido Stříšovský, Radek Šachl

 

Part D - Applications of Fluorescence Spectroscopy to protein studies

Chapter 15

Single-molecule fluorescence spectroscopy of intrinsically disordered proteins

Hagen Hofmannand Wenwei Zheng

 

Chapter 16

Insights into the conformational dynamics of potassium channels using homo-FRET approaches

Ana Coutinho, Clara Díaz-García and María Lourdes Renart

 

Chapter 17

Intrinsic fluorescence kinetics in proteins

Olaf Rolinski and Rhona Muir

 

Chapter 18

Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology

Jan Sykora, Zbynek Prokop, Jiri Damborsky, Abhinav, Martin Hof, Mariana Amaro

 

 

 

 

 

O autorze

Radek Šachl (born in 1982) is a fluorescence spectroscopist and since 2020 head of the Department of Biophysical Chemistry at J. Heyrovský Institute of Physical Chemistry in Prague. He received his Ph.D. in Physical Chemistry at Umeå University in Sweden and at Charles University in Prague in 2012. He did his post-doc at the Royal Institute of Technology in Stockholm. Since 2015 he works at J. Heyrovský Institute of Physical Chemistry, where he was appointed a young scientist position in 2018. His main interest is in development of new fluorescence techniques and their biophysical applications related to lipid membranes and protein-membrane interactions.

Mariana Amaro is a photophysicist by training currently interested in the application of fluorescent techniques in topics linked to proteins and membrane-protein interactions: from relationships between structure-function and aggregation-pathology of proteins to links between activity of membrane receptors and membrane organization/dynamics, while still enjoying the photophysical characterization of novel fluorescent dyes. She obtained her Diploma at Minho University, Portugal, and was awarded her PhD in Physics at University of Strathclyde, Scotland. She joined the J. Heyrovský Institute of Physical Chemistry in 2011 and in 2017 became the Head of the Department of Biophysical Chemistry until she took a maternity break in 2019.