Flow Cytometry Protocols

ISBN-13: 9781493984596 / Angielski / Miękka / 2018 / 492 str.

Teresa S. Hawley; Robert G. Hawley

Spis treści

Dedication…
Preface…
Table of Contents…
Contributing Authors….

1.      Flow Cytometry:- The Glass is Half Full

Howard M. Shapiro

2.      High-Dimensional Modeling for Cytometry: Building Rock Solid Models using GemStone^TM and Verity Cen-se’^TM High-Definition t-SNE Mapping

C. Bruce Bagwell

3.      Mass Cytometry Assays for Antigen-Specific T Cells using CyTOF
Dongxia Lin and Holden T. Maecker

4.      RNA Flow Cytometry using the Branched DNA Technique
Kah Teong Soh and Paul K. Wallace

5.      Analysis of Individual Extracellular Vesicles by Flow Cytometry
John P. Nolan and Erika Duggan

6.      Quantitative Fluorescence Measurements with Multicolor Flow Cytometry
Lili Wang, Adolfas K. Gaigalas, and James Wood

7.      High Throughput Flow Cytometry for Cell Surface Profiling
Joshua Paterson and Laurie E. Ailles

8.      Multiparameter Conventional Flow Cytometry
Katherine M. McKinnon

9.      Multiparameter Intracellular Cytokine Staining
Patricia Lovelace and Holden T. Maecker

10.  Multiparameter Analysis of Apoptosis by Flow Cytometry
William G. Telford

11.  Multiparameter Cell Cycle Analysis
James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan, and Philip G. Woost

12.  Monitoring Cell Proliferation by Dye Dilution: Considerations for Probe Selection
Joseph D. Tario, Jr., Alexis N. Conway, Katharine A. Muirhead, and Paul K. Wallace

13.  Immunophenotypic Identification of Early Myeloerythroid Development
Cornelis J. H. Pronk, and David Bryder

14.  Flow Cytometry Assays in Primary Immunodeficiency Diseases
Maurice R. G. O’Gorman

15.  Real-Time Deformability Cytometry: Label Free Functional Characterization of Cells
Maik Herbig, Martin Kräter, Katarzyna Plak, Paul Müller, Jochen Guck, and Oliver Otto

16.  Nuclear Cytometry: Analysis of the Patterns of DNA Synthesis and Transcription using Flow Cytometry, Confocal Microscopy, and RNA Sequencing
David W. Galbraith, Elwira Sliwinska, and Partha Samadder

17.  Flow Cytometric FRET Analysis of Protein Interactions
László Ujlaky-Nagy, Péter Nagy, János Szöllősi, and György Vereb

18.  Overview of Fluorescence Lifetime Measurements in Flow Cytometry
Jessica P. Houston, Zhihua Yang, Jesse Sambrano, Wenyan Li, Kapil Nichani, and Giacomo Vacca

19.  Overview of Lasers for Flow Cytometry
William G. Telford

20.  Flow Cytometry: The Glass is Half Empty
Howard M. Shapiro

Blurb

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition is a valuable resource for researchers and scientists who are interested in continuing and/or expanding their knowledge of flow cytometry.