Flavoprotein Protocols

ISBN-13: 9781617371585 / Angielski / Miękka / 2010 / 256 str.

Steven K. Chapman; Graeme A. Reid

Opis

As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow--or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g., the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

Recenzje

"A detailed analysis of flavoproteins will be of value for investigators working with flavin enzymes whether studying mechanisms or searching for inhibitors. Chapters on chemical syntheses and the use of analogs provide useful information on approaches to both of these areas.."-Doodys Health Sciences Book Review Journal

Spis treści

UV-Visible Spectroscopy as a Tool to Study Flavoproteins, Peter Macheroux. Identifying and Quantitating FAD and FMN in Simple and in Iron-Sulfur-Containing Flavoproteins, Alessandro Aliverti, Bruno Curti, and Maria Antonietta Vanoni. Fluorescence Analysis of Flavoproteins, Andrew W. Munro and Michael A. Noble. Potentiometric Measurement of Oxidation-Reduction Potential, Stephen G. Mayhew. Flavoprotein Kinetics, Willem J. H. van Berkel, Jacques A. E. Benen, Michel H. M. Eppink, and Marco W. Fraaije. Application of Freeze Quenching to the Study of Rapid Reactions in Flavoproteins, W. John Ingledew. Application of Electron Spin Resonance (ESR) for Detection and Characterization of Flavoprotein Semiquinones, Marat B. Murataliev. Circular Dichroism Studies of Flavoproteins, Andrew W. Munro, Sharon M. Kelly, and Nicholas C. Price. Vibrational Spectroscopy of Flavoproteins, Iain D. G. Macdonald. NMR of Flavoproteins, Jacques Vervoort and Marco Hefti. Flavoprotein Resolution and Reconstitution, Florence Lederer, Heinz Rüterjans, and Garrit Fleischmann. Flavoenzyme Structure and Function: Approaches Using Flavin Analogues, Dale Edmondson and Sandro Ghisla. Identification of Covalent Flavoproteins and Analysis of the Covalent Link, Nigel S. Scrutton. Flavin Synthesis and Incorporation into Synthetic Peptides, R. Eryl Sharp and P. Leslie Dutton. Computational Methods in Flavin Research, Lars Ridder, Hans Zuilhof, Jacques Vervoort, and Ivonne M. C. M. Rietjens. Flavins and Flavoenzymes in Diagnosis and Therapy, Katja Becker, Markus Schirmer, Stefan Kanzok, and R. Heiner Schirmer.