Flavins and Flavoproteins: Methods and Protocols

ISBN-13: 9781493941704 / Angielski / Miękka / 2016 / 468 str.

Stefan Weber; Erik Schleicher

Opis

In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.

Spis treści

Part I

1. Structure and General Properties of Flavins

Ana Maria Edwards

2. Recent advances in Riboflavin Biosynthesis

Ilka Haase, Tobias Gräwert, Boris Illarionov, Adelbert Bacher and Markus Fischer

3. Natural Riboflavin Analogs

Danielle Biscaro Pedrolli, Frank Jankowitsch, Julia Schwarz, Simone Langer, Shinobu Nakanishi and Matthias Mack

4. A Roadmap to the Isotopolog Space of Flavocoenzymes

Adelbert Bacher, Boris Illarionov, Wolfgang Eisenreich and Markus Fischer

5. Electron Transferases

Patricia Ferreira, Marta Martínez-Júlvez and Milagros Medina

6. Aldonolactone Oxidoreductases

Nicole G.H. Leferink and Willem J.H. van Berkel

7. Flavins and Flavoproteins: Applications in Medicine

Esther Jortzik, Lihui Wang, Jipeng Ma and Katja Becker

Part II

8. Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms

Christopher R. Pudney, Sam Hay and Nigel S. Scrutton

9. On the in vivo Redox State of Flavin-Containing Photosensory Receptor Proteins

Aleksandra Bury and Klaas J. Hellingwerf

10. Computational Spectroscopy, Dynamics, and Photochemistry of Photosensory Flavoproteins

Tatiana Domratcheva, Anikό Udvarhelyi, and Abdul Rehaman Moughal Shahi

11. NMR Spectroscopy on Flavins and Flavoproteins

Franz Müller

12. Solid-State NMR of Flavins and Flavoproteins

Anne-Frances Miller

13. EPR on Flavoproteins

Richard Brosi, Robert Bittl, and Christopher Engelhard

14. FTIR Spectroscopy of Flavin-Binding Photoreceptors

Daichi Yamada and Hideki Kandori

15. Resonance Raman Spectroscopy

Jiang Li and Teizo Kitagawa

16. Photoactivation Mechanisms of Flavin-binding Photoreceptors Revealed through Ultrafast Spectroscopy and Global Analysis Methods

Tilo Mathes, Ivo H.M. van Stokkum and John T.M. Kennis

17. Excited State Chemistry of Flavoproteins

Raymond F. Pauszek, Goutham Kodali, and Robert J. Stanley