Fibrous Proteins: Design, Synthesis, and Assembly

ISBN-13: 9781071615737 / Angielski / Twarda / 2021 / 264 str.

Shengjie Ling

Spis treści

Part I: Introduction to Fibrous Proteins

 

1. The Structure of Animal Silks

            Wenwen Zhang and Yimin Fan

 

2. The Structure of Collagen

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

 

3. The Structure of Elastin

            Yuelong Xiao, Shengjie Ling, and Ying Pei

 

4. The Structure of Resilin

            Yuelong Xiao, Shengjie Ling, and Ying Pei

 

5. The Structure of Keratin

            Wenwen Zhang and Yimin Fan

 

Part II: Methods in Synthesis of Fibrous Proteins

 

6. General Methods to Produce and Assemble Recombinant Spider Silk Proteins

            Na Kong

 

7. Self-Assembly of Bombyx mori Silk Fibroin

            Na Kong

 

8. Synthesis and Assembly of Recombinant Collagen

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

 

9. Recombination and Purification of Elastin-Like Polypeptides

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

 

10. Methods to Synthesize and Assemble Recombinant Keratins

            Wenwen Zhang and Yimin Fan

 

11. Preparation of Amyloid Fibers Using Recombinant Technology

            Wenwen Zhang and Yimin Fan

 

12. Molecular Biology Methods to Construct Recombinant Fibrous Protein

            Jicong Zhang

 

Part III: Methods in Assembly of Fibrous Proteins

 

13. Isolation of Nanofibrils from Animal Silks

            Ke Zheng

 

14. Gelation Methods to Assemble Fibrous Proteins

            Ning Fan and Ke Zheng

 

15. Spinning Methods Used for The Construction of One- and Two-Dimensional Fibrous Protein Materials

            Leitao Cao

 

16. Three-Dimensional Printing to Build Fibrous Protein Architectures

            Huanhuan Qiao and Ke Zheng

 

Part IV: Methods in Characterization of Fibrous Proteins

 

17. The Methods of Using Synchrotron FTIR Microspectroscopy to Understand the Conformation of Single Animal Silk Fibers

            Chao Ye, Leitao Cao, and Shengjie Ling

 

18. The Protocol of Using FTIR Imaging to Investigate Silk Fibroin-Based Materials

            Jiajia Zhong, Xiaojie Zhou, Chao Ye, Wenjie Yu, and Yuzhao Tang

 

19. Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy

            Yawen Liu, Hongchong Guo, and Shengjie Ling

 

20. The Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins

            Wenli Gao, Ting Shu, Qiang Liu, Hongchong Guo, Shengjie Ling, and Liang Zhou

 

21. Structural Characterization of Silk Fibers by Wide-Angle X-Ray Scattering

            Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling

 

22. Structural Characterization of Silk Fibers by Small-Angle X-Ray Scattering

            Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling

Blurb

This detailed volume explores fibrous proteins widely present in different biological tissues or biological structural materials. The book begins by introducing the structure of representative fibrous proteins, including animal silks, collagen, elastin, resilin, and keratin, and it then continues by providing detailed experimental protocols for the synthesis, assembly, and characterization of natural, regenerated, and recombinant fibrous proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Fibrous Proteins: Design, Synthesis, and Assembly is an ideal guide for researchers aiming to master fibrous protein preparations with the aid of this broad and interdisciplinary perspective on understanding the structure-property-function relationships of natural and reconstituted fibrous proteins.