Experimental Neurochemistry

ISBN-13: 9781468442106 / Angielski / Miękka / 2013 / 475 str.

Abel Lajtha

Opis

The second volume of the Handbook does not parallel any volume of the first edition; it is one more sign, or reflection, of the expansion of the field. By emphasizing the experimental approach, it illustrates the tools that have re- cently become available for investigating the nervous system. Also, perhaps even more than other volumes, it illustrates the multidisciplinary nature of the field, requiring multidisciplinary methodology. It is now recognized that the availability of methodology is often the rate-limiting determinant of studies and that improvements or innovations in instrumentation can open up new avenues. A new improved method, although opening up new possibilities and being crucial to making advances, is only a tool whose use will determine its use- fulness. If we do not recognize its possibilities, its use will be limited; if we do not recognize its limitations, it will mislead us. It is the possibilities and limitations and the results obtained that are illustrated here.

Spis treści

1 RNA-DNA Hybridization: Analysis of Gene Expression.- 2 Receptor Mapping by Histochemistry.- 3 Receptor Measurement.- 4 Rapid Enzyme Inactivation.- 5 Radioenzymatic Analyses.- 6 Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins.- 7 The Identification of Subcellular Fractions of the CNS.- 8 Cell Isolation.- 9 Principles of Compartmentation.- 10 Diagnosis of Hereditary Neurological Metabolic Diseases.- 11 Human Brain Postmortem Studies of Neurotransmitters and Related Markers.- 12 Neurological Mutants.- 13 Analytical Aspects of the Pharmacokinetics of Psychotrophic Drugs.- 14 Perfusion of the Isolated Brain.- 15 Principles and Application of PET in Neuroscience.- 16 Selected Micromethods for Use in Neurochemistry.- 17 Mass Spectrometric Analysis of Some Neurotransmitters and Their Precursors and Metabolites.- 18 Gas Chromatography.- 19 High-Performance Liquid Chromatography.