Essential Cytometry Methods

ISBN-13: 9780123750457 / Angielski / Miękka / 2009 / 906 str.

Zbigniew Darzynkiewicz

Opis

Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench.

* Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid. * Loaded with detailed protocols developed and used by leaders in the field. *Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology

Spis treści

Part I: Fluorochromes/General Techniques
1. Principles of confocal microscopy
(Robinson)
2. Protein labeling with fluorescent probes
(Holmes, Lantz)
3. Cytometry of fluorescence resonance energy transfer
(Vereb, Matko, Szollosi)
4. The rainbow of fluorescent proteins
(Galbraith)
5. Labeling cellular targets with semiconductor quantum dot conjugates
(Wu, Bruchez)
6. High-gradient magnetic cell sorting
(Radbruch, Mechtold, Thiel, Miltenyi, Pfluger)
7. Multiplexed microsphere assays for protein and DNA binding reactions
(Kellar, Oliver)
8. Biohazard sorting
(Schmid, Roederer, Koup, Ambrozak, Perfetto)
9. Guidelines for the presentation of flow cytometric data.
(Roederer, Darzynkiewicz, Paks)

Part II: Cellular DNA Content Analysis
10. VinDetergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis
(Vindelov,Christensen)
11. Rapid DNA content analysis
(Krishan)
12. DNA analysis from paraffin-embedded blocks
(Hedley)
13. Flow cytometry and sorting of plant protoplasts and cells
(Galbraith)
14. DNA content histogram and cell cycle analysis.
(Rabinovitch)
15. Simultaneous analysis of cellular RNA and DNA content
(Darzynkiewicz)

Part III. Cell Proliferation and Death Assays
16. Immunochemical quantitation of bromodeoxyuridine: application to cell cycle kinetics
(Dolbeare, Selden)

O autorze

Purdue University, W. Lafayette, IN, USA