Epidermal Growth Factor: Methods and Protocols

ISBN-13: 9781617375576 / Angielski / Miękka / 2010 / 208 str.

Tarun B. Patel; Paul J. Bertics

Opis

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."

Recenzje

From the reviews:

"The role of epidermal growth factor (EGF) in both normal development and malignancy has been elucidated gradually over the past half century ... . this book could well prove a great source of help and inspiration to anyone trying to develop kits to simplify and streamline the analysis of the EGF system. ... would also be a good introduction to EGF history, physiology and possible future developments, so it could be worth looking out for in the library ... ." (D. J. Cook, British Journal of Biomedical Science, Vol. 63 (2), 2006)

Spis treści

A Historical Perspective of the EGF Receptor and Related Systems Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. Patel Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. Bertics Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle Analysis of EGF Receptor Interactions With the a Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs Deepti Chaturvedi, Francis Edwin, Hui Sun, and Tarun B. Patel Regulator of Epidermal Growth Factor Signaling: Sprouty Esther Sook Miin Wong and Graeme R. Guy Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway Oliver M. Fischer, Stefan Hart, and Axel Ullrich A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, and Carl P. Blobel In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases Menachem Katz, Yaron Mosesson, and Yosef Yarden Assays to Monitor Degradation of the EGF Receptor Mirko H. H. Schmidt and Ivan Dikic Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members Adriano Marchese Epithelial Cell Migration in Response to Epidermal Growth Factor Reema Zeineldin and Laurie G. Hudson Motility Signaled From the EGF Receptor and Related Systems Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith, and Douglas A. Lauffenburger Methods for Determining the Proliferation of Cells in Response to EGFR Ligands Gregory J. Wiepz, Francis Edwin, Tarun B. Patel, and Paul J. Bertics Clinical Advancement of EGFR Inhibitors in Cancer Therapy Prakash Chinnaiyan and Paul M. Harari Index

Blurb

Epidermal growth factor (EGF) and related molecules regulate a wide array of biological events, ranging from normal growth and development to such pathological states as cancer and cardiovascular diseases. In Epidermal Growth Factor: Methods and Protocols, expert researchers active in the field present their best methods to dissect the mechanisms that control growth factors and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Epidermal Growth Factor: Methods and Protocols offers investigators an array of powerful and proven tools to unravel the workings of epidermal growth factor, as well as develop new strategies for the successful use of this important developmental and therapeutic target.