Enzymology and Molecular Biology of Carbonyl Metabolism 3

ISBN-13: 9781468459036 / Angielski / Miękka / 2012 / 370 str.

David W. Crabb; Henry Weiner;Bendicht Wermuth

Opis

The Fifth International Workshop on the Enzymology and Molecular Biology of Carbonyl Metabolism was held at Purdue University in June, 1990. This represents the fifth time that I had the privilege of organizing the scientific program. It was the first time that I actually hosted the meeting. I wish to salute my four previous co-organizers and the thousands of scientists who have hosted other meetings. It is much easier to arrange the scientific program and edit the proceedings. No local organization could occur without the help of ones research group and, in this case, my wife. I sincerely thank Esther and my research group for their advise and help. At this Workshop, similar to the preceeding ones, much new information was presented. It was apparent how molecular biological techniques were influencing the direction of the research on the three families of enzymes discussed. It also was apparent that not all biochemical problems could be solved by using these techniques. Many of the presentations showed how important advances still could be made using more traditional biochemical approaches.

Spis treści

Aldehyde Dehydrogenase.- Aldehyde Dehydrogenases: What can be Learned From a Baker’s Dozen Sequences?.- Rat Class 3 Aldehyde Dehydrogenase: Crystals and Preliminary Analysis.- Probing the Active Site of Aldehyde Dehydrogenase by Site Directed Mutagenesis.- Localization of Cysteine 302 at the Active Site of Aldehyde Dehydrogenase.- PH Effects on Cytoplasmic Aldehyde Dehydrogenase from Sheep Liver.- Biochemical, Immunological, and Molecular Characterization of a “High KM” Aldehyde Dehydrogenase.- Purification and Properties of Baboon Corneal Aldehyde Dehydrogenase: Proposed UVR Protective Role.- A Polymorphism in the Rat Liver Mitochondrial ALDH2 Gene is Associated with Alcohol Drinking Behavior.- Aldehydic Products of Lipid Peroxidation: Substrates or Inhibitors of Hepatic Aldehyde Dehydrogenase?.- Metabolism of 4-Hydroxynonenal in Hepatoma Cell Lines.- Role of Aldehyde Dehydrogenase (ALDH) in the Detoxication of Cyclophosphamide (CP) in Rat Embryos.- Human and Mouse Hepatic Aldehyde Dehydrogenases Important in the Biotransformation of Cyclophosphamide and the Retinoids.- A Paradigm for Aldehyde Oxidation: Histidinol Dehydrogenase.- Aldo-Keto Reductase.- Structural Studies of Pig Lens Aldose Reductase: Reversible Dimerization of the Enzyme.- cDNA Cloning and Expression of Human Aldose Reductase.- In Vitro Expression of Human Placental Aldose Reductase in Escherichia Coli.- Osmotic Stress Induces Aldose Reductase in Glomerular Endothelial Cells.- Purification of Aldose and Aldehyde Reductases from Dog Kidney.- Study on Dihydrodrodiol Dehydrogenase (I) Molecular Forms of the Enzymes and the Presence of a Dihydrodiol Specific Enzyme in Bovine Liver Cytosol.- Study on Dihydrodiol Dehydrogenase (II) Modulation of Dihydrodiol Dehydrogenase Activity by Biological Disulfides.- Distribution of Dimeric Dihydrodiol Dehydrogenase in Pig Tissues and its Role in Carbonyl Metabolism.- Inhibition of Aldehyde Reductase by Carboxylic Acids.- Reactivity of Enzyme Modification Reagents with Aldose Reductase and Aldehyde Reductase.- A Kinetic Perspective on the Peculiarity of Aldose Reductase.- Best-Fit Analysis of Kinetic Scheme for the Stepwise Reduction of the “Diketo” Group of 6-Pyruvoyl Tetrahydropterin by Sepiapterin Reductase.- Alcohol Dehydrogenase.- Catalysis by Yeast Alcohol Dehydrogenase.- Alcohol Dehydrogenase Gene Expression, and Cloning of the Mouse chi(x)-Like ADH….- Role of Alcohol Dehydrogenase Polymorphism in Ethanol Metabolism and Alcohol-Related Diseases.- Characterization of Human Alcohol Dehydrogenases Containing Substitutions at Amino Acids 47 and 51.- Endocrine Regulation and Methylation Patterns of Rat Class I Alcohol Dehydrogenase in Liver and Kidney.- Mammalian Class II Alcohol Dehydrogenase: Species and Class Comparisons at Genomic and Protein Levels.- The Activation of Alcohols by Liver Alcohol Dehydrogenase: Dependence of Inhibition upon the pKa Lowering Effect.- Glutathione-Dependent Formaldehyde Dehydrogenase (EC 1.2.1.1): Evidence for the Identity with Class III Alcohol Dehydrogenase.- Kinetics and Mechanism of Methanol and Formaldehyde Interconversion and Formaldehyde Oxidation Catalyzed by Liver Alcohol Dehydrogenase.- Novel Substrates and Inhibitors of Human Liver Sorbitol Dehydrogenase.- Comparison of the Primary Structures of NAD(P)-Dependent Bacterial Alcohol Dehydrogenases.- Contributors.