Enzymes in RNA Science and Biotechnology Part B

ISBN-13: 9780443239762 / Twarda / 2023 / 412 str.

Spis treści

Post-transcriptional modification enzymes
1. Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA
Li-Sheng Zhang
2. Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA
Li-Sheng Zhang
3. Discovering RNA modification enzymes using a comparative genomics approach
Satoshi Kimura
4. Functional analysis of tRNA modification enzymes using mutational profiling
Ryota Yamagami and Hiroyuki Hori
5. Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins
Ute Kothe and Sarah Schultz
6. Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis
Howard Gamper, Caroline McCormick, Amr Makhamreh, Meni Wanunu, Sara Rouhanifard and Ya-Ming Hou

Nucleases
7. Characterizing RNase L-mediated mRNA decay in single cells
James Burke, Renee Cusic and Monty Watkins
8. Characterization of RNase J
Balasubramanian Gopal, Muralidharan Vandanashree and Ankur Singh
9. Pri-miRNA cleavage assays for the Microprocessor complex
Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
10. The pre-miRNA cleavage assays for DICER
Tuan Anh Nguyen, Cong Truc Le and Thi Nhu Y. Le
11. Methods for study of ribonuclease targeting chimeras
Matt Disney, Noah Springer, Samatha Meyer, Yuquan Tong, Jessica Childs-Disney and Raphael Isaac Benhamou
12. Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus
Winton Wu, Chi Nam Ignatius Pang, Jai Tree and Daniel Giuseppe Mediati