Enzyme Stabilization and Immobilization: Methods and Protocols

ISBN-13: 9781607618942 / Angielski / Twarda / 2010 / 229 str.

Shelley D. Minteer

Opis

In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.

Spis treści

Enzyme Stabilization and Immobilization: Methods and Protocols Table of Contents: Table of Contents Preface Contributors 1. Introduction to the field of enzyme immobilization and stabilization – Michael J. Moehlenbrock and Shelley Minteer 2. Stabilization of enzymes through encapsulation in liposomes- Makoto Yoshimoto 3. Micellar enzymology for thermal, pH, and solvent stability – Shelley Minteer 4. Lipase activation and stabilization in room temperature ionic liquids – Joel L. Kaar 5. Nanoporous silica glass for the immobilization of interactive enzyme systems – Andreas Buthe, Songtao Wu, and Ping Wang 6. Enzyme stabilization and immobilization by sol-gel entrapment – Allen E. David, Arthur J. Yang, and Nan Sun Wang 7. Nanoporous Gold for Enzyme Immobilization – Keith J. Stine, Kenise Jefferson, and Olga V. Shulga 8. Enzyme stabilization via bio-templated silicification reactions – Glenn R. Johnson and Heather R. Luckarift 9. The immobilization of enzyme on Eupergit supports by covalent attachment – Zorica D. Knezevic-Jugovic, Dejan I. Bezbradica, Dusan Z. Mijin, and Mirjana G. Antov 10. Micellar polymer encapsulation of enzymes – Sabina Besic and Shelley D Minteer 11. Enzyme Stabilization via Cross-Linked Enzyme Aggregates – Munishar Gupta and Smita Raghava 12. Enzyme immobilization in polyelectrolyte microcapsules – Michael J. McShane 13. Macroporous poly(GMA-co-EGDMA) for enzyme stabilization – Nenad Milosavic and Radivoje M. Prodanovic 14. Enzyme-nanoparticle conjugates for biomedical applications – Alexey A. Vertegel, Vladimir Reukov, and Victor Maximov 15. Enzyme nanoparticle fabrication- Magnetic nanoparticle synthesis and enzyme immobilization - Patrick Johnson, Hee Joon Park, and Ashley J. Driscoll 16. Cytochrome C stabilization and immobilization in aerogels – Amanda S. Harper-Leatherman, Jean Marie Wallace, and Debra R. Rolison 17. Kinetic Measurement for Enzyme Immobilization – Michael Cooney