Electroporation Protocols: Preclinical and Clinical Gene Medicine

ISBN-13: 9781617378201 / Angielski / Miękka / 2010 / 524 str.

Shulin Li

Opis

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology(TM) series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Spis treści

Dec 1st, 2006 Editor: Shulin Li (USA) Book title: Electroporation protocols: Experimental and Clinical Gene Medicine Publisher: The Humana Press, Inc Preface (Shulin Li, no need to fit chapter format) I. Basic concepts of DNA transfer via electroporation (review chapters, no need to fit chapter format for all the chapters in this section) Application of Electroporation gene therapy: past, current, and future (Lluis M. Mir) Mechanism by which electroporation mediates DNA migration and entry into cells and the targeted tissues (Marie-Pierre Rols) Applicator and electrode design for in vivo DNA delivery by electroporation (Dietmar Rabussay) Electrode assemblies used for electroporation of cultured cells (Leda Raptis and Kevin L. Firth) Formulations for DNA delivery via electroporation in vivo (Khursheed Anwer) Overview of drug delivery and alternative methods to electroporation (Sek-Wen Hui) Nano-Electroporation—A First Look (Raji Sundararajan) II. In vitro targeted gene delivery via electroporation Nucleofection of Human Embryonic Stem Cells (Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, and Oliver Brustle) Delivery of whole tumor lysate into dendritic cells for cancer vaccination (Linda N. Liu, Rama Shivakumar, Cornell Allan, and Joseph C. Fratantoni) Delivery of tumor antigen encoding mRNA into dendritic cells for vaccination (Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, and Kris Thielemans) Delivery of DNA into natural killer cells for immunotherapy (Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg) Electroporation ofadherent cells in situ for the study of signal transduction and gap junctional communication (Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, and Kevin L. Firth) Delivery of DNA into adipocytes within adipose tissue (James G. Granneman) III: In vivo targeted gene delivery via electroporation Delivery of DNA into muscle for treating systemic diseases: advantage and challenge (review chapter, no need to fit chapter format) (Capucine Trollet, Daniel Scherman, Pascal Bigey) Delivery DNA into skeletal muscle in large animals (Patricia A. Brown, Amir S. Khan, and Ruxandra Draghia-Akli) Delivery DNA into skin via electroporation (Babu M. Medi and Jagdish Singh) Electroporation-mediated Gene Delivery to the Lungs(Rui Zhou, James E. Norton, and David A. Dean) Delivery DNA into bladder via electroporation (Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, and Akito Inadome) Analysis of gene function in the retina (Takahiko Matsuda and Constance L. Cepko) Optical in vivo imaging of electrically mediated delivery of siRNA into muscle for gene function analysis (Muriel Golzio and Justin Teissie) Electroporation of adult zebrafish (N. Madhusudhana Rao, K. Murali Rambabu, and S. Harinarayana Rao) IV: Treatment of cancer via electroporation gene therapy Flow electroporation with pulsed electric fields for purging tumor cells (Abie Craiu and David Scadden) Delivery of DNA into tumors (Shulin Li) Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumor in Dogs (Jeffry Cutrera, Marina Torrero, Keijiro Shiomitsu, Neal Mauldin, and Shulin Li)

Blurb

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields.  In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease.  Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.