Dna-Protein Interactions: Principles and Protocols, Third Edition

ISBN-13: 9781617378751 / Angielski / Miękka / 2010 / 680 str.

Tom Moss; Beno T. LeBlanc

Opis

Gene expression can mean the difference between health and disease, the difference between survival and starvation. This third edition brings this vital subject up to date with protocols exploring the most cutting-edge developments in the field.

Recenzje

Reviews of Previous Editions:

"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology

"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science

Spis treści

1. Filter-Binding Assays Peter G. Stockley 2. Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions Manon Gaudreault, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc, and Sylvain L. Guérin 3. DNase I Footprinting Benoît Leblanc and Tom Moss 4. Exonuclease III Footprinting on Immobilized DNA Templates Patrizia Spitalny and Michael Thomm 5. Hydroxyl Radical Footprinting of Protein-DNA Complexes Indu Jagannathan and Jeffrey J. Hayes 6. The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA Brenda F. Kahl and Marvin R. Paule 7. Uranyl Photofootprinting Peter E. Nielsen 8. Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference Peter E. Shaw and A. Francis Stewart 9. Ethylation Interference Footprinting of DNA-Protein Complexes Iain W. Manfield and Peter G. Stockley 10. Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates within Model Chromatin Complexes David R. Chafin and Jeffrey J. Hayes 11. Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX Philippe Bouvet 12. Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting Simon Labbé, Jean-François Harrisson, and Carl Séguin 13. Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper Athanasios G. Papavassiliou 14. Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots Athanasios G. Papavassiliou 15. Use ofa Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions David R. Setzer, Deborah B. Schulman, Cathy V. Gunther, and Michael J. Bumbulis 16. Chromatin Immunoprecipitation in Mammalian Cells Amy Svotelis, Nicolas Gévry, and Luc Gaudreau 17. Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP Mayra Furlan-Magaril, Héctor Rincón-Arano, and Félix Recillas-Targa 18. Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae Alain R. Bataille and François Robert 19. Nucleosome Mapping Nicolas Gévry, Amy Svotelis, Marc Larochelle, and Luc Gaudreau 20. In Cellulo DNA Analysis (LMPCR Footprinting) Régen Drouin, Nathalie Bastien, Jean-François Millau, François Vigneault, and Isabelle Paradis 21. Atomic Force Microscopy Imaging and Probing of DNA, Proteins and Protein-DNA Complexes: Silatrane Surface Chemistry Yuri L. Lyubchenko, Luda S. Shlyakhtenko, and Alexander A. Gall 22. Two-Dimensional Crystallisation of Soluble Protein Complexes Patrick Schultz, Corinne Crucifix, and Luc Lebeau 23. Assays for Transcription Factor Activity Douglas Browning, Nigel Savery, Annie Kolb, and Stephen Busby 24. Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine Rainer Meffert, Klaus Dose, Gabriele Rathgeber, and Hans-Jochen Schäfer 25. Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes Nikolai Naryshkin, Sergei Druzhinin, Andrei Revyakin, Younggyu Kim, Vladimir Mekler, and Richard H. Ebright 26. Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription

Blurb

Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, DNA-Protein Interactions: Principles and Protocols, Third Edition serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research.