1. Survival of pathogens on surfaces and the influence of inoculating matrix on survival capabilities
Matthew J. Igo and Donald W. Schaffner
2. Enumeration of viable cells of bacteria in food and water with flow cytometry
Jossana Pereira de Sousa Guedes, and Evandro Leite de Souza
3. Evaluation of physiological characteristics of bacterial cells in foods and water with flow cytometry
Jossana Pereira de Sousa Guedes, and Evandro Leite de Souza
4. Detection of sublethally injured cells by the selective medium plating technique
Elisa Pagán, Daniel Berdejo, Natalia Merino, and Diego García-Gonzalo, Rafael Pagán
5. Combining culturing technique and metabarcoding to study microbiota in the meat industry
Natalia Merino, Elisa Pagán, Daniel Berdejo, Rafael Pagán, and Diego García-Gonzalo
6. Assessment of in vitro biofilms by plate count and crystal violet staining: is one technique enough?
Elisa Gascón, Natalia Merino, Elisa Pagán, Daniel Berdejo, Rafael Pagán, and Diego García-Gonzalo
7. Evolution assays for the isolation of mutant bacteria resistant to natural antimicrobials
Daniel Berdejo, Elisa Pagán, Natalia Merino, and Diego García-Gonzalo, Rafael Pagán
8. Preparing yeast suspension through serial dilution for enumeration
Cíntia Lacerda Ramos and Karina Teixeira Magalhaes-Guedes
9. Standardizing suspension of yeast for inoculation in food fermentations
Luara Aparecida Simões, Angélica Cristina de Souza, Rosane Freitas Schwan, and Disney Ribeiro Dias
10. Enumerating yeast in foods and water using the spread plating technique
Angélica Cristina de Souza, Luara Aparecida Simões, Rosane Freitas Schwan, and Disney Ribeiro Dias
11. Enumerating distinct yeast in the same food sample
Luara Aparecida Simões, Angélica Cristina de Souza, Rosane Freitas Schwan, and Disney Ribeiro Dias
12. Detection and quantification of yeast species in food samples for quality control
Cíntia Lacerda Ramos and Karina Teixeira Magalhaes-Guedes
13. Evaluation of yeast inoculated in parallel to the autochthonous microbiota in food samples
Maria Gabriela Cruz Pedrozo Miguel, Luara Aparecida Simões, Disney Ribeiro Dias, and Rosane Freitas Schwan
14. Double layer plaque assay technique for enumeration of viruses surrogates
Ruthchelly Tavares da Silva, Maria Mayara de Souza Grilo, Marciane Magnani, and Geany Targino de Souza Pedrosa
15. Detection of protozoan parasites on leafy greens using multiplex PCR
Minji Kim and Karen Shapiro
16. Viability of Trypanosoma cruzi in food and beverages
Rodrigo Labello Barbosa and Karen Signori Pereira
17. Detection of Giardia cysts and Cryptosporidium oocysts in edible shellfish: choosing a target
Diego Averaldo Guiguet Leal, Taís Rondello Bonatti, Roberta de Lima, Rodrigo Labello Barbosa, and Regina Maura Bueno Franco
18. Protocol for the detection of Toxoplasma gondii oocysts in water samples
Felippe Danyel Cardoso Martins, Winni Alves Ladeia, Fernanda Pinto-Ferreira, Italmar Teodorico Navarro, and Roberta Lemos Freire
19. Detection of Toxoplasma gondii in milk and cheese
Fernanda Pinto-Ferreira, Felippe D. C. Martins, Winni A. Ladeia, and Italmar T. Navarro
20. Detection of Toxoplasma gondii in meat
Winni A. Ladeia, Felippe D. C. Martins, Fernanda Pinto-Ferreira, Roberta L. Freire, and Italmar T. Navarro
21. Detection of Toxoplasma gondii and Cyclospora cayetanensis in oysters
Minji Kim and Karen Shapiro
This volume details methods and procedures used to detect and enumerate bacteria in food. Chapters guide readers through food and beverage matrices, techniques used to enumerate bacteria, mixed bacterial strains (naturally present or inoculated), yeast, viruses, protozoan in distinct food matrices, and freshwater.
Authoritative and cutting-edge, Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater aims to provide a basic understanding on detection and enumeration of microorganisms in foods.