DNA Topoisomerase Protocols: Volume I: DNA Topology and Enzymes

ISBN-13: 9781617370397 / Angielski / Miękka / 2010 / 327 str.

Mary-Ann Bjornsti; Neil Osheroff

Opis

Beginning with the Escherichia coli co protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes has been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytofoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Volume I; DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.

Recenzje

"...an invaluable practical aid. The central and expanding role of these enzymes in basic cell biology together with their important pharmaceutical applications will ensure that book will remain relevant for some years to come."-Cell Biology International 2000

"The introductory chapter by the editors gives a beautifully brief and concise overview of the entire family of DNA topoisomerases and related enzymes and can be used as an Ariadne's thread to the key papers of the field. Another useful item is the appended compendium of topoisomerase sequences, which is likely to be helpful in designing expression vectors, deletion mutants, etc. Most of the other chapters contain a lot of useful information and easily followed procedures.a very valuable addition to the topoisomerase literature. It summarizes a lot of useful information for those engaged or getting engaged in research on DNA topology or topoisomerases. Those just wanting to carry out some of the analytical procedures will in most cases find what they need, i.e. a concise delineation of the analytical background, some detailed and easy to follow protocols, and a comprehensive guideline for the interpretation of the results. As such, the book definitely deserves a place on many laboratory shelves"-FEBS Letters

"The introductory chapter is one of the most concise and clearly presented summaries of our current knowledge that has been written, and it will be useful even for experts in the field....The volume concludes with a well-organized and useful compendium of toposomerase DNA sequences. Volume I is clearly a valuable source of methodological expertise for anyone studying topoisomerases."-New Biological Books

"...We recommend greatly this remarkable book to all our readers, without exception." - Cellular and Molecular Biology

Spis treści

Introduction to DNA Topoisomerases, Mary-Ann Bjornsti and Neil Osheroff. Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis,Mary-Ann Bjornsti and Maureen D. Megonigal. Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers, Ryo Hanai and Joaquim Roca. Analysis of Altered DNA Structures: Cruciform DNA, Albert J. Courey. Purification of Supercoiled Plasmid DNA, Kelly L. Jordan, Devon L. Evans, and David J. Hall. Purification and Use of DNA Minicircles with Different Linking Numbers, Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, and Ernesto Di Mauro. Isolation of Kinetoplast DNA, Theresa A. Shapiro, Viiu A. Klein, and Paul T. Englund. Isolation of Knotted DNA from Coliphage P4, Morten Isaksen, Bryan Julien, Richard Calendar, and Björn H. Lindqvist. Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis, Stephen D. Levene and Hua Tsen. Sedimentation Analysis of Bacterial Nucleoid Structure, Karl Drlica, Chang-Rung Chen, and Samuel Kayman. Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy, E. Lynn Zechiedrich and Nancy J. Crisona. Methods for Analyzing DNA Bending, Jason D. Kahn. Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination, Marc R. Gartenburg. Overexpression and Purification of Bacterial DNA Gyrase, Anthony Maxwell and Alison J. Howells. Overexpression and Purification of Bacterial DNA Topoisomerase I, Chang-Xi Zhu and Yuk-Ching Tse-Dinh. Overexpression and Purification of Escherichia coli DNA Topoisomerase III, Russell J. DiGate. Overexpression and Purification of Bacterial DNA Topoisomerase IV, Hong Peng and Kenneth J. Marians. Purification of Bacteriophage T4 Type II DNA Topoisomerase, Kenneth N. Kreuzer and Sue H. Neece. Overexpression and Purification of DNA Topoisomerase I from Yeast,Mary-Ann Bjornsti and Jolanta Fertala. Overexpression and Purification of Saccharomyces cerevisiae DNA Topoisomerase II from Yeast, Janet E. Lindsley.Purification of DNA Topoisomerase II from Drosophilamelanogaster, Stacie J. Froelich-Ammon, Paul S. Kingma, and Neil Osheroff. Purification of DNA Topoisomerase I from Human Placenta, Joseph A. Holden. Purification of Baculovirus-Expressed Human DNA Topoisomerase I, Lance Stewart and James J. Champoux. Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography, Harald Biersack, Sanne Jensen, and Ole Westergaard. Metabolic Labeling, Immunoprecipitation, and Two-Dimensional Tryptic Phosphopeptide Mapping of Human Topoisomerase II, Nicholas J. Wells and Ian D. Hickson. Immunoblot Analysis and Band Depletion Assays, Scott H. Kaufmann and Phyllis A. Svingen. Visualization of DNA Topoisomerases by Electron Microscopy, Piero Benedetti and Alessandra Silvestri. Appendix: Compendium of DNA Topoisomerase Sequences, Paul R. Caron.

Blurb

DNA Topoisomerase Protocols, I: DNA Topology and Enzymes brings together an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, and bacterial, yeast and mammalian expression systems. A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.

DNA Topoisomerase Protocols, I: DNA Topology and Enzymes will be of immense value to the many basic scientists and clinicians who want better to understand and to exploit proficiently the wealth of recent discoveries about chromatin structure and its relation to gene expression, about DNA topoisomerases as the targets of antitumor and antibacterial agents, and about DNA repair.