DNA Sequencing Protocols

ISBN-13: 9781617371509 / Angielski / Miękka / 2011 / 256 str.

Colin A. Graham; Alison J. M. Hill

Opis

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Recenzje

"DNA Sequencing Protocols covers the commonly used protocols in a clearly written manner and is thorough in description. This book is highly recommended for all libraries serving researchers involved in any aspect of DNA sequencing." - E-STREAMS

Spis treści

Introduction to DNA Sequencing, Colin A. Graham and Alison J. M. Hill. The Universal Primers and the Shotgun DNA Sequencing Method, Joachim Messing. M13 Sequencing, Qingzhong Yu. Primer Design and Primer-Directed Sequencing, Ulrike Gerischer and Peter Dürre. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction, Ulrike Gerischer. Solid Phase Fluorescent Sequencing of the CFTR Gene, Harry Cuppens and Jean-Jacques Cassiman. Shotgun DNA Sequencing, Alan T. Bankier. Cycle Sequencing, Robert W. Blakesley. Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers, Johannes Zschocke and Georg F. Hoffmann. Automated Fluorescent DNA Sequencing on the ABI PRISM 377, Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer, David Watts and Jonathan R. E. MacBeath. Fluorescent Sequencing Protocols for the ALF, Shirley McQuaid. Fluorescent Sequencing for Heterozygote Mutation Detection, Colin A. Graham and Alison J. M. Hill. Sequence Databases and the Internet, Anne E. Hughes. DNA Sequencing by Capillary Array Electrophoresis, Norman J. Dovichi and JianZhong Zhang. Index.

Blurb

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project.
State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.