DNA Sequencing Protocols

ISBN-13: 9781489942128 / Angielski / Miękka / 2013 / 244 str.

Colin A. Graham; Alison J. M. Hill

Opis

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Recenzje

From Reviews of the First Edition

"...continues the standard of excellent laboratory manuals that has been set by earlier volumes...Many of the protocols are significantly easier to follow than comparable protocols in other lab handbooks...provides one of the best collections of manuals for DNA sequencing applications..." -FEBS Letters
"...a valuable addition to the library of any laboratory that is actively engaged in DNA sequencing..." -Quarterly Review of Biology
"...any laboratory undertaking a large DNA sequencing project...would benefit from having this manual..." -Trends in Biotechnology

Spis treści

DNA SEQUENCING PROTOCOLS, Second Edition Edited by Colin A. Graham and Alison J. M. Hill Contents 1. Introduction to DNA sequencing Colin A. Graham and Alison J. M. Hill 2. M13 cloning Joachim Messing 3. M13 sequencing Qingzhong Yu 4. Primer Design and Primer directed sequencing Ulrike Gerischer and Peter Dürre 5. Direct sequencing of DNA produced in a Polymerase Chain Reactio Ulrike Gerischer 6. Solid phase fluorescent sequencing of the CFTR gene Harry Cuppens and Jean-Jacques Cassiman 7. Shotgun sequencing Alan T. Bankier 8. Cycle sequencing Robert W. Blakesley 9. Cycle Sequencing of PCR-Amplified Genomic DNA with Dye-Labelled Universal Primers Johannes Zschocke and Georg F. Hoffmann 10. Automated fluorescent DNA sequencing on the ABI PRISM 377 Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd 11. Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyser David Watts and Jonathan R. E. MacBeath 12. Fluorescent sequencing protocols for the ALF Shirley MacQuaid 13. Fluorescent sequencing for heterozygote mutation detection Colin A. Graham and Alison J. M. Hill 14. Automation in DNA sequencing Alan T. Bankier 15. Sequence databases and the internet Anne E. Hughes Introduction to DNA Sequencing. M13 Cloning. M13 Sequencing. Primer Design and Primer Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene Shotgun Sequencing. Cycle Sequencing. Cycle Sequencing of PCR-Amplified Genomic DNA With Dye-Labelled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyser. Fluorescent Sequencing Protocols for the ALF Fluorescent Sequencing for Heterozygote MutationDetection. Automation in DNA Sequencing. Sequence Databases and the Internet.

Blurb

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project.
State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.