DNA Repair Protocols: Prokaryotic Systems

ISBN-13: 9781617371127 / Angielski / Miękka / 2010 / 192 str.

Pat Vaughan

Opis

When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its Molecule of the Year in 1994."

Recenzje

"...must for any science library supporting a molecular biology program." - E-Streams

"An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie

Spis treści

Part I. Assays to Detect and Quantitate DNA Repair Activity. Repair of A/G and A/8-oxoG Mismatches by MutY Adenine DNA Glycosylase, A-Lien Lu. Assays for the Repair of Oxidative Damage by Formamidopyrimidine Glycosylase (Fpg) and 8-) Oxoguanine DNA Glycosylase (OGG-1), Amanda J. Watson and Geoffrey P. Margison. Detection and Quantitation of Uracil DNA Glycosylase Activity, Geraldine M. O'Grady. Detection of DNA Deoxyribophosphodiesterase Activity, Margarita Sandigursky and William A. Franklin. O6-Alkylguanine-DNA Alkytransferase Assay, Amanda J. Watson and Geoffrey P. Margison. The E. coli Vsr Endonuclease: Assaying Activity In Vitro and In Vivo, Claire G. Cupples and Georgina Macintyre. Coupling of DNA Helicase Function to DNA Strand Exchange Activity, Frank G. Harmon and Stephen C. Kowalczykowski. Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity, Douglas A. Julin. Holliday Junction Branch Migration and Resolution: RuvA, RuvB, and RuvC from the Hyperthermophile Thermotoga maritima, Susana Gonzalez and James G. Wetmur. MutS-DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance, Peter Brooks. Part II. Use of DNA Repair Enzymes as Tools in Molecular Biology. Flexible Genetic Engineering Using RecA Protein, Lance J. Ferrin. Interspecies Recombination and Mismatch Repair: Generation of Mosaic Genes and Genomes, Ivan Matic, François Taddei, and Miroslav Radman. The Use of Immobilized Mismatch Binding Protein in Mutation/SNP Detection, Robert Wagner and Alan Dean. Use of Uracil DNA Glycosylase in the Detection of Known DNA Mutations and Polymorphisms: Glycosylase-Mediated Polymorphism Detection (GMPD-Check), Pat Vaughan. Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms: Glycosylase-Mediated Polymorphism Detection (GMPD-Scan), Pat Vaughan. The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection, Jeffrey J. Babon, Matthew McKenzie, and Richard G. H. Cotton.Index.

Blurb

DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information.
Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.