Cytotoxic T-Cells: Methods and Protocols

ISBN-13: 9781493911578 / Angielski / Twarda / 2014 / 301 str.

Elena Ranieri

Opis

​ In Cytotoxic T-Cells: Methods and Protocols, leading experts in the field provide a collection of state-of-the art methods and protocols encompassing a wide array of systems biology approaches for Cytotoxic T-Cell research. Dived into three main sections, the first part of the volume analyzes the isolation of T Cells along with their expansion and characterization according to different methods. The second part describes required techniques for intracellular signaling, monitoring of antigen T cell specific responses, CTL exosomes, and microscopy and in vivo imaging applied to CTL studies. The final section focuses on specialist applications of molecular methods into the study of CTL, including next generation sequencing of the Jack/stat pathway and CTL involvement in bone remodeling and transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cytotoxic T-Cells: Methods and Protocols seeks to aid scientists in the further study into concepts of laboratory methods using systems biology.

Spis treści

1. Negative And Positive Separation Techniques For The Isolation Of Antigen-Specific Cd8⁺ T Cells From Blood And Tumor Tissue

Margherita Gigante, Sharon Natasha Cox, and Elena Ranieri

 

2. In Vitro\Ex Vivo Generation Of Cytotoxic T Lymphocytes

Margherita Gigante  and Elena Ranieri

 

3. Phenotypic And Functional Characterization Of Cytotoxic T Lymphocytes By Flow Cytometry

Lulia Popescu, Matthew Pipeling, Jason Akulian,  and John Mcdyer

 

4. Intracellular Signalling Of Ctls

Sharon Natasha Cox

 

5. Monitoring Antigen-Specific T Cell Responses Using Real-Time Pcr

Devin B. Lowe, Jennifer L. Taylor, And Walter J. Storkus

 

6. Ctl Elispot Assay

Elena Ranieri, Iulia Popescu, and Margherita Gigante

 

7. Methods Of Purification Of Ctl-Derived Exosomes

Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli

 

8. Characterization Of Ctl By Microscopy

Giuseppe Stefano Netti

 

9. Intravital Imaging Of Cytotoxic T Lymphocytes

Gaetano Faleo, Per-Olof Berggren, and Antonello Pileggi

 

10. The Microarray-Based Approach For The Analysis Of The Transcriptome

Matteo Accetturo, Paola Pontrelli, and Loreto Gesualdo

 

11. Mirnome Analysis Using Real Time Pcr

Paola Pontrelli, Matteo Accetturo, and Loreto Gesualdo

 

12. Proteomic Approaches By Seldi And Maldi-Tof/Ms For Ctl Analysis

Massimo Papale and Maria Teresa Rocchetti

 

13. Two-Dimensional Gel Electrophoresis Approach For Ctl Phosphoproteome Analysis

Maria Teresa Rocchetti, Massimo Papale, and Loreto Gesualdo

 

14. Targeting  The Jak/Stat Pathway In Cytotoxic T Lymphocytes (Ctl) By Next Generation Sequencing (Ngs)

Maddalena Gigante, Sterpeta Diella, and Elena Ranieri

15. Experimental Model For Studying The Involvement Of Regulatory Cytotoxic T Cells In Bone Resorption

Giacomina Brunetti, Angela Oranger, Silvia Colucci, and Maria Grano

 

16. Ctl And Transplantation: Tissue In Vivo Characterization

Federica Rascio, Chiara Divella, and Giuseppe Grandaliano