Cytokinesis: Volume 137

ISBN-13: 9780128096734 / Angielski / Twarda / 2017 / 480 str.

Arnaud Echard

Opis

Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.

• Covers sections on cytokinesis and emerging studies
• Presents chapters written by experts in the field
• Includes cutting-edge materials that supplement study

Spis treści

1. Labeling Membrane Domains in Dividing Echinoderm Eggs David Burgess 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis Toshihide Kobayashi 3. Analysis of Protein Dynamics During Cytokinesis in Budding Yeast Erfei Bi 4. Imaging cytokinesis of Drosophila S2 cells Gilles Hickson 5. Studying cytokinesis in Drosophila epithelial tissues Yohanns Bellaïche 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes Maria Grazia Giansanti 7. Monitoring complete and incomplete abscission in the germline stem cell lineage of Drosophila ovaries J R. Huyhn 8. Incomplete Cytokinesis / Binucleation in Mammals: the powerful system of Hepatocytes. Chantal Desdouets 9. Post-abscission membrane dynamics associated with cytokinesis - basal process splitting and midbody release Wieland B. Huttner 10. Understanding the role of cytokinesis and midbody formation during epithelial cell polarization Rytis Prekeris 11. Micromanipulation of daughter cells for the study of cytokinetic abscission Matthieu Piel 12. Measuring Abscission Spatiotemporal Dynamics using Quantitative High Resolution Microscopy Natalie Elia 13. Analysis of cytokinesis by electron microscopy Thomas Müller-Reichert 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM Arnaud Echard 15. Analysis of Post-cytokinetic Roles of Cytokinetic Components in C. elegans Guangshuo Ou 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using C. elegans Amy Shaub Maddox 17. Using fast-acting temperature sensitive mutants to study cell division in C. elegans Julie C. Canman 18. Cytokinesis from nanometers to micrometers and microseconds to minutes Douglas N. Robinson 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis Ewa Kamila Paluch 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast Sophie Martin 21. Single molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers Martin Loose 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GAP subunits for in vitro structural and functional assays Masanori Mishima 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells Issei Mabuchi 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis Christine Field

O autorze

Arnaud Echard received a PhD in Molecular and Cell Biology from the University of Paris in 2000. In the lab of Dr. Bruno Goud at the Institut Curie, he discovered and characterized the first molecular motor of the kinesin superfamily localized at the Golgi apparatus. During his post-doctoral training at UCSF, USA in Pr. Pat O'Farrell Lab, he then worked on cell division in Drosophila embryos and carried out one of the first genome-wide RNAi-based screen designed to discovered conserved genes essential for cytokinesis.

In 2003, he has been hired at the French National Center for Scientific Research (CNRS) first at the Institut Curie, then at the Institut Pasteur. There, he set up the lab "Membrane Traffic and Cell Division” in the Cell Biology and Infection department in 2010.

Since 2006, he is part-time Professor of Molecular and Cellular Biology at the Ecole Polytechnique, France.

He has been awarded a Schlumberger Foundation Prize for Education and Research in 2010 and a Medical Research Foundation award in 2011. He has also been elected Vice-President (2012-2016) of the French Society for Cell Biology (SBCF) and President of the society since 2016, representing 180 labs and 1200 members.