Cytokine Protocols

ISBN-13: 9781493958108 / Angielski / Miękka / 2016 / 241 str.

Marc de Ley

Opis

The new edition of this Methods in Molecular Biology(TM) book covers techniques for studying cytokines: bioassay, quantification and characterization of cytokine related RNA, natural and artificial posttranscriptional RNA modifications and protein-level observation.

Spis treści

1    A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery

Alfons Billiau

2   The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels

Maria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes

3     Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID

Tomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki

4   Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues

Markus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil  

5 Evaluating Posttranscriptional Regulation of Cytokine Genes

Bernd Rattenbacher and Paul R. Bohjanen

6    Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay

Latifa Al-Haj and Khalid S.A. Khabar

7    Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo

Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka

8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor

Paulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz

^{9 Control of the Interferon Response in RNAi Experiments}

^{Jana Nejepinska, Matyas Flemr, and Petr Svoboda}

 ^10.  shRNA-induced Interferon-Stimulated Gene Analysis

Núria Morral and Scott R. Witting

11   Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells

Fabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik

^{12   Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins}

^{Daniela Novick and Menachem Rubinstein}

13        In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells

William C. Adams and Karin Loré 

 

14        A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells

Lennart Zabeau, José Van der Heyden, and Jan Tavernier

Blurb

Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology.  These advancements have allowed researchers to  reduces sample sizes making for more accurate determinations then previously possible. In Cytokine Protocols: Second Edition, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs,  posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research.