Cytochrome P450 Protocols

ISBN-13: 9781627033206 / Angielski / Twarda / 2013 / 319 str.

Ian R. Phillips; Elizabeth A. Shephard; Paul R. Ortiz De Montellano

Opis

Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs ('de-orphanizing' CYP substrates). Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.

Recenzje

From the reviews of the third edition:

"With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. ... this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions. ... This stands alone as the premier book for this field, which is sorely lacking in publications of this type." (Jed N. Lampe, Doody's Book Reviews, October, 2013)

Spis treści

1. Bioluminescent Assays for Cytochrome P450 Enzymes

            Douglas S. Auld, Henrike Veith, and James J. Cali

 

2. In Vitro Drug-Drug Interaction Assay      

            Michael Zientek and Kuresh Youdim

 

3. High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening

            Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel

 

4. The Synthesis, Characterization and Application of ¹³C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites

            Christopher McCullough, Phani Kumar Pullela, Sang-Choul Im, Lucy Waskell, Daniel Sem

 

5. High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators    

            Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg

 

6. Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches

            Qian Cheng and F. Peter. Guengerich

 

7. Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis

            Hugues Ouellet, Eric D. Chow, Shenheng Guan, Jeffery S. Cox, Alma L. Burlingame, and Paul R. Ortiz de Montellano

 

8. An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins  

            Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta

 

9. Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group

            Wesley E. Straub, Clinton R. Nishida, and Paul R. Ortiz de Montellano

 

10. Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes     

            A. Luthra, M. Gregory, Y.V. Grinkova, I.G. Denisov , and S.G. Sligar

 

11. Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles

            Linlin Zhao, Besnik Bajrami, and James F. Rusling

 

12. Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells

            M. Teresa Donato and M. José Gómez-Lechón

 

13. Screening for Cytochrome P450 Reactivity with a Reporter Enzyme

            Kersten S. Rabe and Christof M. Niemeyer

 

14. High-Throughput Fluorescence Assay of Cytochrome P450 3A4          

            Qian Cheng and F. Peter. Guengerich

 

15. Targeted Protein Capture for Analysis of Electrophile-Protein Adducts

            Rebecca E. Connor, Simona G. Codreanu, Lawrence J. Marnett, and Daniel C. Liebler

 

16. DNA Shuffling of Cytochrome P450 Enzymes

            James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam

 

17. Measurement of P450 Difference Spectra Using Intact Cells

            Wayne A. Johnston and Elizabeth M.J. Gillam

 

18. DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production

            Nedeljka N. Rosic

 

19. P450 oxidoreductase – Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein

            Vishal Agrawal and Walter L. Miller

 

20. LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450

            Federico Sabbadin, Gideon G. Grogan and Neil C. Bruce

 

21. Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database

            Sarah C. Sim and Magnus Ingelman-Sundberg

 

22. Simultaneous in vivo Phenotyping of CYP Enzymes

            Sussan Ghassabian and Michael Murray

 

23. Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting

            Matthew Hancock and Elizabeth A. Shephard

 

24. Isolation of Mouse Hepatocytes

            Mina Edwards, Lyndsey Houseman, Ian R. Phillips, and Elizabeth A. Shephard

 

25. Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies

            Véronique Laurent, Denise Glaise, Tobias Nübel, David Gilot, Anne Corlu, and Pascal Loyer