Cyclic Nucleotide Signaling in Plants: Methods and Protocols

ISBN-13: 9781493962914 / Angielski / Miękka / 2016 / 273 str.

Chris Gehring

Opis

This book details approaches to better understand signal transduction and the molecular mechanisms underlying cellular signaling. It features step-by-step, readily reproducible laboratory protocols as well as lists of the necessary materials and reagents.

Spis treści

1: Molecular Methods for the Study of Signal Transduction in Plants

Helen R. Irving and Chris Gehring

 

2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi

 

3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Heike Burhenne and Volkhard Kaever

  

4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts

Richard M. Graeff and Hon Cheung Lee

 

5: In vivo Imaging of cGMP in Plants

Jean-Charles Isner and Frans J.M. Maathuis

 

 6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses

Fouad Lemtiri-Chlieh and Rashid Ali

 

7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides

Natalia Maria Ordoñez, Lana Shabala, Chris Gehring, and Sergey Shabala

 

 8: Calcium Imaging of the Cyclic Nucleotide Response

Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya

 

 

9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation

Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze

 

 

10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules

Claudius Marondedze, Kathryn Lilley and Ludivine Thomas

11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome

Lara Donaldson and Stuart Meier

  

12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity

Victor Muleya, Janet I. Wheeler and Helen R. Irving

  

13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants

Aloysius Wong and Chris Gehring

  

14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions

Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz

  

15: Inferring biological functions of guanylyl cyclases with computational methods

May Alqurashi and Stuart Meier

 

16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases

Erin B. Purcell and Rita Tamayo

  

17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants

Robin K. Walker and Gerald A. Berkowitz

 

18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin

Ndiko Ludidi

 

 19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses

Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito

 

 

Blurb

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.