Correlative Light and Electron Microscopy: Volume 111

ISBN-13: 9780124160262 / Angielski / Twarda / 2012 / 460 str.

Thomas Mueller-Reichert

Opis

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

• Covers many areas of CLEM by the best international scientists in the field
• Includes a brief history and overview on CLEM methods

Spis treści

1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
2. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy
3. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
4. Correlative Light and Electron Microscopy in Parasite Research
5. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
6. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
7. Correlative Light and Electron Microscopy of GFP
8. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
9. Correlated Light Microscopy and Electron Microscopy (CLEM): Search. and Find!
10. Capturing Endocytic Segregation Events with HPF-CLEM
11. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
12. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
13. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
14. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
15. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
16. Atmospheric Scanning Electron Microscope for Correlative Microscopy
17. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
18. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

O autorze

Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.

TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.
He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. His focus is on the study of sorting mechanisms in intracellular transport pathways and in the area of Synthetic Biology. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 85 papers and edited 4 books on CLEM (including 3 Volumes of the Methods in Cell Biology series).
PV obtained his PhD on electron microscopic studies of the peripheral nervous system at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001.
He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He has acted as chair and co-chair of the Electron Microscopy section of the Royal Microscopical Society and is closely involved with BioimagingUK shaping the UK imaging infrastructure landscape.
He has organised and taught on several courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. His lab is the home of the EMBO practical course on CLEM and he is Work Group leader for CLEM within the EU COST project COMULIS (COrrelated MUltimodal imaging in LIfe Sciences).