Confocal Microscopy: Methods and Protocols

ISBN-13: 9781588293510 / Angielski / Twarda / 2013 / 381 str.

Paddock, Stephen W.

Opis

Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.

Spis treści

1. Reflecting on Confocal Microscopy: A Personal Perspective

            John White

 

2.  Laser Scanning Confocal Microscopy: History, Applications And Related Optical Sectioning Techniques

            Stephen W. Paddock and Kevin W. Eliceiri

 

3. Confocal Microscopy on the Internet

            Kristin L. Hazelwood, Christopher S. Murphy, Zachary C. Cunningham, Matthew J. Parry-Hill, Richard L. Ludlow, Ericka B. Ramko, Anna P. Ozarowska, Adam M. Rainey and Michael W. Davidson

 

4. Using Photoshop with Images Created by a Confocal System

            Jerry Sedgewick

 

5. Clearing up the Signal: Spectral Imaging and Linear Unmixing in Fluorescence Microscopy

            Timo Zimmermann, Joanne Marrison, Karen Hogg, and Peter O’Toole

 

6. Low Magnification Confocal Microscopy of Tumor Angiogenesis

            George McNamara, Anna Yanai, Vazgen Khankaldyyan, Walter E. Laug, Jeff Boden, Keith Webster, Yiwen Li, and Rong Wen

 

7. Confocal Imaging of Butterfly Tissue

            Craig R. Brunetti

 

8. Confocal Microscopy of Cardiac Myocytes

            Robert L. Price, Stephen T. Haley, Tara Bullard, Jeffrey Davis, Thomas K. Borg, and Louis Terracio

 

9. Confocal Imaging of Fluorescently Labeled Proteins in the Drosophila Larval Neuromuscular Junction

            Ian P. Coyle

 

10. Confocal Imaging and Three-Dimensional Visualization of Thick Autofluorescent Specimens

            Angela V. Klaus, Valerie Schawaroch, and Kevin J. Frischmann

 

11. Vital Imaging of Multicellular Spheroids

            Paulo J. Oliveira, Ed Perkins, and Jon Holy

 

12. Live Confocal Analysis of Mutant and Drug Treated Drosophila Embryos

            Barbara Fasulo and William Sullivan

 

13. Confocal Imaging of the Microtubule Cytoskeleton in C. elegans Embryos and Germ Cells

            Kevin F. O’Connell and Andy Golden

 

14. Measurement in the Confocal Microscope

            Guy Cox

 

15. A Method for Quantifying Blood Flow Distribution Among The Alveoli of the Lung

            Robert L. Conhaim, Dennis M. Heisey, and Greg E. Leverson

 

16. Imaging Tools for Analysis of the Ureteric Tree in the Developing Mouse Kidney

            Luise A. Cullen-McEwen, Richard J. Young, Gabriel Fricout, Dominique Jeulin, Ian S. Harper, Frank Costantini, and John F. Bertram

 

17. Evaluating Confocal Microscopy System Performance

            Robert M. Zucker