Colorectal Cancer: Methods and Protocols

ISBN-13: 9781617371790 / Angielski / Miękka / 2010 / 294 str.

Steven M. Powell

Opis

The contents of Colorectal Cancer: Methods and Protocols aim to instruct investigators in all the key genetic, cellular, and molecular biological methods of analyzing colorectal tumors. The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench. Of note, most of the chapters in this volume are written by those scientists who p- neered these methods and assays in their respective fields. The chapters in Colorectal Cancer: Methods and Protocols describe state of the art methods to analyze colorectal tumors, ranging from gross mic- dissection of specimens to specific molecular analyses. Included are coverages of mutational assays, instability testing, immunohistochemical assays, chro- somal studies, and gene expression analyses. The goal of our volume is to facilitate the performance of colorectal tumor biological experiments by investigators at various levels of training from graduate students and postdoctoral fellows to principal investigators who desire to advance our understanding of colon cancer development."

Recenzje

"This is quite interesting and a valuable volume. It aims to instruct investigators in the key genetic, cellular and molecular biological methods for analyzing colorectal tumors. It's quite precise in its description of these techniques and, as the preface says, "The focused techniques and assays are described in sufficient detail to allow researchers to start an experiment on colon tumors and proceed from beginning to end as if the expert in the field who has performed these studies were guiding them at the bench." A large amount of practical information is packed into one small volume and this volume is ideal for students and fellows who wish to expand their knowledge in this area and have a ready volume reference in developing new techniques...The volume is also good value for those of us who don't get into the lab as it clarifies many of the techniques which are used and helps the non-involved reader to understand the terminology and techniques used in this important area of cancer research." - Cancer Forum

"...Dr. Saltz has produced as concise a presentation as possible within the framework of the widely scattered disciplines searching the complexity of diagnostic and therapeutic problems occurring among patients with colorectal cancer." - Comprehensive Therapy

Spis treści

Microdissection of Histological Sections: Manual and Laser Capture Microdissection Techniques Christopher A. Moskaluk Isolation of a Purified Epithelial Cell Population from Human Colon James K. Roche Xenografting Human Colon Cancers Jeffrey C. Harper, Reid B. Adams, and Steven M. Powell Comparative Genomic Hybridization Technique Wa'el El-Rifai and Sakari Knuutila Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics Svetlana D. Pack and Zhengping Zhuang Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer Jin Jen The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity Darryl Shibata Microsatellite Instability Testing Yann R. Parc and Kevin C. Halling Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer Kevin C. Halling and Patrick C. Roche Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes Julie M. Cunningham, David J. Tester, and Stephen N. Thibodeau Mutation Detection in the TGF-b Receptors William M. Grady, Lois L. Myeroff, Hongmei He, and Sanford Markowitz Direct Analysis for Familial Adenomatous Polyposis Mutations Steven M. Powell I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer Perry S. Chan and Steven L. Gersen Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas Rhonda F. Souza and Stephen J. Meltzer Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors Sam Thiagalingam Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations Lauri A. Aaltonen and Stina Roth Direct Sequencing for Peutz-Jehgers Gene LKB1 (STK11) Mutations Lauri A. Aaltonen and Egle Avizienyte Direct Sequencing for Cowden Syndrome Gene PTEN (MMAC1) Mutations Lauri A. Aaltonen, Stina Roth, and Charis Eng Genetic Analysis Using Microarrays Wa'el El-Rifai and Sakari Knuutila Cyclooxygenase-2 (COX-2) Protein Expression by Western Blotting Hossam M. Kandil and Raymond N. DuBois The Immunohistochemical Method Lisa A. Cerilli and Henry F. Frierson, Jr Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids Rosana Cosme and James K. Roche Adhesion Signaling Through Integrins Reid B. Adams and Joshua D. Rovin Electrophoretic Mobility Shift Assay (EMSA) Michael F. Smith, Jr. and Sandrine Delbary-Gossart Transfection Assays for Transformation of Model Colonic Cell Lines Mark T. Worthington and Roger Qi Luo Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay Kimberly S. Tustison, Joanne Yu, and Steven M. Cohn Gene Transfer into the Colonic Mucosa Baçak Çoruh and Theresa T. Pizarro Index

Blurb

Colorectal cancer is a common and highly lethal cancer once spread whose treatment is improving as we gain a better understanding of its development. In Colorectal Cancer: Methods and Protocols, Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying this solid tumor. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Also presented are mutational analyses of colon tumors and blood samples to determine whether specific patients have inherited a significant predisposition to colorectal cancer. Each method is described in step-by-step detail by an established expert to ensure robust experimental results, and all are accompanied by detailed notes on pitfalls to avoid.
Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.