Collagen: Methods and Protocols

ISBN-13: 9781493990948 / Angielski / Twarda / 2019 / 254 str.

Sagi, Irit

Opis

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Spis treści

Part I: In Vitro Models for Collagen Characterization

 

1. Cloning and Mutagenesis Strategies for Large Collagens

            Olivier Bornert and Alexander Nyström

 

2. Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen

            Venkat Raghavan Krishnaswamy

 

3. Production and Characterization of Chemically Cross-Linked Collagen Scaffolds

            Ignacio Sallent, Héctor Capella-Monsonís, and Dimitrios I. Zeugolis

 

4. A Biomimetic Model for Mineralization of Type-I Collagen Fibrils

            Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A.J.M. Sommerdijk, and Ruikang Tang

 

Part II: Proteomics Analysis of Collagen

 

5. Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy

            Wing Ying Chow

 

6. Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry

            Amar Joshi, Amna Zahoor, and Alberto Buson

 

7. Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes

            Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, and Matthew D. Shoulders

 

8. Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics

            Elizabeta Madzharova, Fabio Sabino, and Ulrich auf dem Keller

 

Part III: Imaging Approaches for Collagen

 

9. Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy

            Elee Shimshoni and Irit Sagi

 

10. In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology

            Yang Li and S. Michael Yu

 

11. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics

            Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokládal, Florence Ruggiero, Jean-Marc Allain, and Marie-Claire Schanne-Klein

 

12. Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix

            Uwe Hansen

 

Part IV: Collagen in Health and Disease

 

13. Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans

            Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, and Collin Y. Ewald

 

14. Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis

            Efrat Kessler and Eyal Hassoun

 

15. Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment

            Chen Varol

 

16. FACS Analysis of Col1α Protein Levels in Primary Fibroblasts

            Noam Cohen and Neta Erez

 

17. Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors

            Gregg B. Fields