Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols

ISBN-13: 9781493931811 / Angielski / Twarda / 2015 / 333 str.

Uttam Garg

Opis

Clinical Applications of Mass Spectrometry in Biomolecular Analysis

Recenzje

"This is a compendium of mass spectrometry protocols for clinically important analytes. ... This book is intended for anyone interested in clinical applications of mass spectrometry. It would be of interest to basic scientists, clinical laboratory practitioners (e.g., scientists, physicians, etc.), manufacturers, and healthcare providers interested in applications of mass spectrometry. ... This is your book if you are contemplating implementing mass spectrometry in your laboratory for any of these clinically important analytes." (Valerie L. Ng, Doody's Book Reviews, March, 2016)

Spis treści

1. Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
Uttam Garg and Y. Victoria Zhang

2. Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
David Scott, Bryce Heese, and Uttam Garg

3. Quantification of Arginine and its Methylated Derivatives in Plasma by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Faye B. Vicente, Gina Vespa, Alan Miller, and Shannon Haymond

4. Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
Hemamalini Ketha and Ravinder J. Singh

5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography -Tandem Mass Spectrometry (LC-MS/MS)
J. Grace Van Der Gugten and Daniel T. Holmes

6. Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-Column Double Derivatization Method
Shuang Deng, David Scott, and Uttam Garg

7. Sensitive, Simple, and Robust Nano Liquid Chromatography-Mass Spectrometry Method for Amyloid

Protein Subtyping
Drew Payto, Courtney Heideloff, and Sihe Wang

8. Quantitation of Ubiquinone (Coenzyme Q₁₀) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
Richard E. Mathieu Jr., and Catherine P. Riley

9. Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
Yan Victoria Zhang

10. Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
Shuang Deng, David Scott, Douglas Myers, and Uttam Garg

11. Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
Catherine P. Riley, Richard E. Mathieu Jr., and Carmen Wiley

12. Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
Julie A. Ray, Mark M. Kushnir, Alan L. Rockwood, and A. Wayne Meikle

13. Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry
Erland Arning and Teodoro Bottiglieri

14. Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
J. Grace Van Der Gugten, Sophia Wong, and Daniel T. Holmes

15. Quantitation of Insulin Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
Hemamalini Ketha and Ravinder J. Singh

16. Quantitation of Free Metanephrines in Plasma by Liquid Chromatography Tandem Mass Spectrometry
Courtney Heideloff, Drew Payto, and Sihe Wang

17. Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography Tandem Mass Spectrometry
Jessica Gabler and Sihe Wang

18. High Throughput Analysis of Methylmalonic Acid in Serum, Plasma and Urine by LC-MS/MS: Method for Analyzing Isomers without Chromatographic Separation
Mark M. Kushnir, Gordon J. Nelson, Elizabeth L. Frank, and Alan L. Rockwood

19. Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry
Erland Arning and Teodoro Bottiglieri

20. Quantitative

Organic Acids in Urine by Two Dimensional Gas Chromatography Time of Flight Mass Spectrometry (GCxGC-TOFMS)
Lawrence Sweetman, Paula Ashcraft, and Jeanna Bennett-Firmin

21. High Sensitivity Measurement of Pancreatic Polypeptide and its Variant in Serum and Plasma by LC-MS/MS
Hernando Escobar, Mark M. Kushnir, Alan L. Rockwood, and A. Wayne Meikle

22. Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography Tandem Mass Spectrometry
Hemamalini Ketha and Ravinder J. Singh

23. Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography Tandem Mass Spectrometry
Judy Peat and Uttam Garg

24. Urine Purine Metabolite Determination by UPLC Tandem Mass Spectrometry
Qin Sun

25. Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
Qin Sun

26. Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography -Tandem Mass Spectrometry (LC-MS/MS)
J. Grace Van Der Gugten and Daniel T. Holmes

27. Quantitation of

S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry
Erland Arning and Teodoro Bottiglieri

28. A Simple, High Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
Wei-Lien Chuang, Joshua Pacheco, and Kate Zhang

29. Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone and Testosterone by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)
Ada Munar, Clint Frazee, and Uttam Garg

30. Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
Hongjie Chen and Chunli Yu

31. Quantification of 1,25-Dihydroxyvitamin D2 & D3 in Serum Using Liquid Chromatography Tandem Mass Spectrometry
Jonathon Mahlow, Dustin R. Bunch, and Sihe Wang

32. High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
Judy Stone

33. Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in Urine Using LC-MS/MS
Dean C. Carlow, Ryan C. Schofield, and M. Denburg

 

Blurb

This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.