Clinical Applications of Capillary Electrophoresis: Methods and Protocols

ISBN-13: 9781493992126 / Angielski / Twarda / 2019 / 273 str.

Terry M. Phillips

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Overview

1. An Overview of CE in Clinical Analysis

David S. Hage

Part II Applications in Clinical Chemistry

2. Rapid and Sensitive Determination of Branched-Chain Amino Acids in Human Plasma by Capillary Electrophoresis with Contactless Conductivity Detection for Physiological Studies

Petr Tůma

3. Monitorization of α1-Acid Glycoprotein Deglycosylation using SU-8 Microchips Electrophoresis with LIF Detection

María del Mar Barrios-Romero, Agustín G. Crevillén, Angel Puerta, Mercedes de Frutos, and José Carlos Diez-Masa

4. Glycoform Analysis of Alpha1-Acid Glycoprotein by Capillary Electrophoresis using Electrophoretic Injection

Chenhua Zhang, William Clarke, and David S. Hage

5. On-Line Immunoaffinity Solid-Phase Extraction Capillary Electrophoresis-Mass Spectrometry for the Analysis of Serum Transthyretin

Roger Pero-Gascon, Laura Pont, Victoria Sanz-Nebot, and Fernando Benavente

6. Measurement of Neutral and Sialylated IgG N-glycome at Asn-297 by CE-LIF to Assess Hypogalactosylation in Rheumatoid Arthritis

Christian Schwedler and Véronique Blanchard

7. The Control of Glucose and Lactate Levels in Nutrient Medium after Cell Incubation and in Microdialysates of Human Adipose Tissue by Capillary Electrophoresis with Contactless Conductivity Detection

Petr Tůma

8. Flow-Induced Dispersion Analysis (FIDA) for Protein Quantification and Characterization 

Morten E. Pedersen, Jesper Østergaard, and Henrik Jensen

Part III Application in Drug Analysis

9. A Chiral Generic Strategy for Enantioseparation of Acidic and Basic Drugs using Short End Injection Capillary Electrophoresis: Application to Design of Experiment

Hassan Y. Aboul-Enein and Ahmed M. Abdel-Megied 

Part IV Application in Metabolomics

10. New Advances for Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Mass Spectrometry (CE-MS)

Meera Shanmuganathan and Philip Britz-McKibbin

11. Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of Biomass-Limited Sample

Wei Zhang, Thomas Hankemeier, and Rawi Ramautar

Part V Applications in Paediatrics

12. Device Fabrication and Fluorescent Labeling of Preterm Birth Biomarkers for Microchip Electrophoresis

Anna V. Nielsen and Adam T. Woolley

13. Analysis of Inflammatory Mediators in New-Born Dried Blood Spot Samples by Chip-Based Immunoaffinity Capillary Electrophoresis

Terry M. Phillips and Edward F. Wellner

14. Triplet-Repeat Primed PCR and Capillary Electrophoresis for Characterizing the Fragile X Mental Retardation 1 CGG Repeat Hyperexpansions

Indhu-Shree Rajan-Babu and Samuel S. Chong

Part VI Applications in Oncology

15. A Capillary Electrophoresis UV Detection-Based Method for Global Genomic DNA Methylation Assessment in Human Whole Blood

Angelo Zinellu, Elisabetta Sotgiu, Salvatore Sotgia, and Ciriaco Carru

16. Capillary Electrophoresis Analysis of Prostate Specific Antigen (PSA)

Noemi Farina-Gomez, Diana Navarro-Calderon, Angel Puerta, Monica Gonzalez, Jose Carlos Diez-Masa, and Mercedes de Frutos

17. Prostate Protein N-Glycoylation Profiling by Means of DNA Sequencer-Assisted Fluorophore-Assisted Carbohydrate Electrophoresis

Tijl Vermassen, Nico Callewaert, Sylvie Rottey, and Joris R. Delanghe

 

18. Aptamer-Based Microchip Electrophoresis Assays for Amplification Detection of Carcinoembryonic Antigen

Shulin Zhao

Part VII Application in Virology

19. Highly Sensitive SDS Capillary Gel Electrophoresis with Sample Stacking Requiring Only Nanograms of Adeno-Associated Virus Capsid Proteins 

Chao-Xuan Zhang and Michael M. Meagher 

Blurb

This second edition volume provides a valuable source of information on the application of capillary electrophoresis (CE) and the many different aspects of clinical medicine. Chapters divided into seven parts focus on applications in clinical chemistry and small molecule analysis, applications in drug analysis, examples of CE applied to metabolomics, application in pediatrics, CE analysis on oncology, and CE analysis in virology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Clinical Applications of Capillary Electrophoresis: Methods and Protocols, Second Edition aims to become a resource not only for clinical chemists, but also physicians and scientists who wish to apply these techniques to diagnosis and clinical research.