Circulating Micrornas: Methods and Protocols

ISBN-13: 9781627034524 / Angielski / Twarda / 2013 / 270 str.

Nobuyoshi Kosaka

Opis

microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside."

Spis treści

1. Functional Analysis of Exosomal MicroRNA in Cell-Cell Communication Research

Nobuyoshi Kosaka, Yusuke Yoshioka, Keitaro Hagiwara, Naoomi Tominaga and Takahiro Ochiya

 

2.  Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture 

Takayuki Kogure and Tushar Patel

 

3. Methods of Analysis of Dendritic Cell-derived Exosome-shuttle MicroRNA and its Horizontal Propagation Between Dendritic Cells

Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morell

 

4. Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse

Carolina Villarroya-Beltri, Cristina Gutiérrez-Vázquez, Francisco Sánchez-Madrid, and María Mittelbrunn

 

5. Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo

Frederik J Verweij, Monique AJ van Eijndhoven, Jaap Middeldorp and D Michiel Pegtel

 

6. Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells

Tian Sheng Chen and Sai Kiang Lim

 

7. Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction

Steven J. Greco, Pranela Rameshwar

 

8. Isolation of Circulating MicroRNA Associated with RNA-binding Protein

Andrey Turchinovich, Ludmila Weiz,  and Barbara Burwinkel

 

9. Identification and Analysis of Circulating Exosomal microRNA in Human Body Fluids

Cecilia Lässer

 

10. Analyzing the Circulating MicroRNAs in Exosomes/Extracellular Vesicles from Serum or Plasma by qRT-PCR

Leni Moldovan, Kara Batte, Yijie Wang, Jon Wisler, and Melissa Piper

 

11. Direct Serum Assay for MicroRNA in Cancer Patients

Sota Asaga  and Dave S.B. Hoon

 

12. Analysis of MicroRNA Niches: Techniques to Measure Extracellular MicroRNA and Intracellular MicroRNA In Situ

Victoria N. Parikh and Stephen Y. Chan

 

13. Analysis of Circulating MicroRNA by Microarray in Liver Disease

Yoshiki Murakami and Toshihito Tanahashi

 

14. Isolation of Circulating MicroRNA in Saliva

Alessia Gallo, Ilias Alevizos

 

15. Purification of RNA From Milk Whey

Hirohisa Izumi, Nobuyoshi Kosaka, Takashi Shimizu, Kazunori Sekine, Takahiro Ochiya, and Mitsunori Takase

 

16. Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases

Akira Machida, Takuya Ohkubo, and Takanori Yokota

 

17. Methods of MicroRNA Quantification in Urinary Sediment

Wang Gang, and Szeto Cheuk Chun,

 

18. Circulating microRNA for the Identification of Forensically Relevant Body Fluids

Erin K. Hanson and Jack Ballantyne

 

19. Identification of Prostate Cancer-associated MicroRNAs in Circulation using a Mouse Model of Disease

Luke A. Selth, Scott Townley, Joanna Gillis, Wayne Tilley and Lisa Butler

 

20. A Combination of Extraction Reagent and DNA Microarray that Allows for the Detection of Global MiRNA Profiles from Serum /Plasma

Makiko Ichikawa and Hideo Akiyama

 

21. Nanopore Single-molecule Detection of Circulating microRNAs

Li-Qun Gu and Yong Wang