Chemosensitivity: Volume II: In Vivo Models, Imaging, and Molecular Regulators

ISBN-13: 9781588295866 / Angielski / Twarda / 2005 / 442 str.

Rosalyn D. Blumenthal; Rosalyn D. Blumenthal

Opis

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individuals. These readily reproducible techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor

Spis treści

Part I. Measurements of DNA Damage, Cell Death, and Regulators of Cytotoxicity In Vitro Micronucleus Technique to Predict Chemosensitivity Michael Fenech Cell Cycle and Drug Sensitivity Aslamuzzaman Kazi and Q. Ping Dou TUNEL Assay as a Measure of Chemotherapy-Induced Apoptosis Robert Wieder Apoptosis Assessment by the DNA Diffusion Assay Narendra P. Singh PARP Cleavage and Caspase Activity to Assess Chemosensitivity Alok C. Bharti, Yasunari Takada, and Bharat B. Aggarwal Diphenylamine Assay of DNA Fragmentation for Chemosensitivity Testing Cicek Gercel-Taylor Immunodetecting Members of the Bcl-2 Family of Proteins Richard B. Lock and Kathleen M. Murphy Correlation of Telomerase Activity and Telomere Length to Chemosensitivity Yasuhiko Kiyozuka Application of Silicon Sensor Technologies to Tumor Tissue In Vitro: Detection of Metabolic Correlates of Chemosensitivity Pedro Mestres-Ventura, Andrea Morguet, Anette Schofer, Michael Laue, and Werner Schmidt Overview of Tumor Cell Chemoresistance Mechanisms Laura Gatti and Franco Zunino Flow Cytometric Monitoring of Fluorescent Drug Retention and Efflux Awtar Krishan and Ronald M. Hamelik Flow Cytometric Measurement of Functional and Phenotypic P-Glycoprotein Monica Pallis and Emma Das-Gupta Measurement of Ceramide and Sphingolipid Metabolism in Tumors: Potential Modulation of Chemosensitivity David E. Modrak Part II. Genomics, Proteomics, and Chemosensitivity Gene Expression Profiling to Characterize Anticancer Drug Sensitivity James K. Breaux and Gerrit Los Identifying Genes Related to Chemosensitivity Using Support Vector Machine Lei Bao Genetic Manipulation of Yeast to Identify Genes Involved in Regulation of Chemosensitivity Giovanni L. Beretta and Paola Perego Real-Time RT-PCR (Taqman®) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouracil Tetsuro Kubota Use of Proteomics to Study Chemosensitivity Julia Poland, Silke Wandschneider, Andrea Urbani, Sergio Bernardini, Giorgio Federici, and Pranav Sinha Part III. In Vivo Animal Modeling of Chemosensitivity Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivity Lee Su Kim and Janet E. Price Orthotopic Metastatic (MetaMouse®) Models for Discovery and Development of Novel Chemotherapy Robert M. Hoffman Preclinical Testing of Antileukemic Drugs Using an In Vivo Model of Systemic Disease Richard B. Lock, Natalia L. Liem, and Rachael A. Papa Assessing Growth and Response to Therapy in Murine Tumor Models C. Patrick Reynolds, Bee-Chun Sun, Yves A. DeClerck, and Rex A. Moats Evaluation of Chemosensitivity of Micrometastatses with Green Fluorescent Protein Gene-Tagged Tumor Models in Mice Hayao Nakanishi, Seiji Ito, Yoshinari Mochizuki, and Masae Tatematsu 99mTc-Annexin A5 Uptake and Imaging to Monitor Chemosensitivity Tarik Z. Belhocine and Francis G. Blankenberg Magnetic Resonance Imaging of Tumor Response to Chemotherapy Richard Mazurchuk and Joseph A. Spernyak Metabolic Monitoring of Chemosensitivity with 18FDG PET Guy Jerusalem and Tarik Z. Belhocine Index

Blurb

Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe their best laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains today's best protocols for classifying tumors into response categories and for customizing therapy to individuals. These techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. Highlights include genomic and proteomic approaches to assess chemosensivity, in vivo imaging approaches to assess early response to therapy, and methods to statistically analyze data from in vivo therapy. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.
Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more or less sensitive to a particular agent.