Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part a: Volume 621

ISBN-13: 9780128181171 / Angielski / Twarda / 2019 / 382 str.

Arun K. Shukla

Recenzje

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Spis treści

1. Targeted m⁶A reader proteins to study the epitranscriptome Simone Rauch and Bryan C. Dickinson 2. Synthetic gene expression circuits regulating sexual reproduction Nobuo Fukuda and Shinya Honda 3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA Justin M. Thomas, Keri M. Bryson and Jordan L. Meier 4. A guide for drug inducible genome editing with HIT systems Chen Zhao, Shixian Wei and Yu Wang 5. A guide for drug inducible transcriptional activation with HIT systems Chen Zhao, Shixian Wei and Yu Wang 6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore 7. Salt-sensitive intein for large-scale polypeptide production Yi-Zong Lee and Shih-Che Sue 8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg 9. Design, cloning and characterization of transcription factor-based inducible gene expression systems Erik K.R. Hanko, Nigel P. Minton and Naglis Malys 10. Design, construction, and validation of optogenetic proteins Colin P. O'Banion, Anwesha Goswami and David S. Lawrence 11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg 12. Chemical biology of glycoproteins: From chemical synthesis to biological impact Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan 13. In bulla functional channel expression systems that mimic bacterial synthetic membranes Masayuki Iwamoto and Shigetoshi Oiki 14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems John E. Gadbery and Nicole S. Sampson 15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin 16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia 17. Improved sensitivity and resolution of in-cell NMR spectra David S. Burz, Leonard Breindel and Alexander Shekhtman 18. High-throughput methods in aptamer discovery and analysis Kyle H. Cole and Andrej Lupták 19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki

O autorze

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla's research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.