Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C: Volume 633

ISBN-13: 9780128191286 / Angielski / Twarda / 2020 / 308 str.

Arun K. Shukla

Recenzje

"Given that the preceding Parts A and B of this series were both published nearly a decade ago (i.e., 2011), Part C offers a timely overview of recent technological advances and novel findings in these rapidly moving areas of research and development. This most recent addition to the prestigious Methods in Enzymology series complements, rather than competes with, the numerous books available on these topics, which generally focus on either synthetic or chemical biology or specific aspects thereof (e.g., "Inorganic Chemical Biology" or "Systems Biology Application in Synthetic Biology"), but not both, and rarely offer the detailed descriptions of specific methods and procedures found in this book." --Doody

Spis treści

1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein Elke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen 2. (Strept)avidin as a template for ligands other than biotin: An overview Vesa P. Hytönen 3. Engineering siderophores Sina Rütschlin and Thomas Böttcher 4. Competitive profiling for enzyme inhibitors using chemical probes Michaela Prothiwa and Thomas Böttcher 5. The NMR tube bioreactor Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos 6. A chemically-controlled system for activating RAS GTPases Emily M. Dieter and Dustin J. Maly 7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9 Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler 8. Synthetic receptors to understand and control cellular functions Hung-Ju Chang and Jerome Bonnet 9. A suite of bioassays to evaluate CREB inhibitors Bingbing X. Li and Xiangshu Xiao 10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe Xiangshu Xiao and Bingbing X. Li 11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins Marcus J. C. Long, Daniel A. Urul and Yimon Aye 12. Building artificial genetic circuits to understand protein function Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham 13. Methods for studying human sirtuins with activity-based chemical probes Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen 14. Site-directed labeling of ß-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla 15. Reversible biotinylation of purified proteins for measuring protein-protein interactions Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla

O autorze

Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla's research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.