Chaperones

ISBN-13: 9781071633410 / Angielski / Twarda / 2023

Opis

This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chaperones: Methods and Protocols, Second Edition is a valuable resource for all researchers who want to learn more about this interesting and developing field. 

Spis treści

Dedication…
Acknowledgement…
Preface…
Table of Contents…
Contributing Authors…

1. Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Andrew Ackerman, Toshiki Kijima, Takanori Eguchi, and Thomas L. Prince

2. Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays
Heyoun Bunch

3. Role of Heat Shock Factors in Stress-Induced Transcription: An Update
Heyoun Bunch and Stuart K. Calderwood

4.. A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
Kristina M. Holton, Richard M. Giadone, Benjamin J. Lang, and Stuart K. Calderwood

5. Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
Ritwick Sawarkar

6. Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Network
Yuka Okusha and Stuart K. Calderwood

7. Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug Resistance
Xiongjie Jin, Demetrius Moskophidis, and Nahid F. Mivechi

8. Bimolecular Fluorescence Complementation Assay to Evaluate HSP90-Client Protein Interactions in Cells
Abir Chakraborty, Gregory L. Blatch, and Adrienne L. Edkins

9. Complementation Assays for Co-Chaperone Function
Adrienne L. Edkins and Gregory L. Blatch

10. Optimized Microscale Protein Aggregation Suppression Assay: A Method for Evaluating the Holdase Activity of Chaperones
Ronald Tonui, Ruth O. John, and Adrienne L. Edkins

11. Detecting Post-Translational Modifications of Hsp90 Isoforms
Rebecca A. Sager, Sarah J. Backe, Len Neckers, Mark R. Woodford, and Mehdi Mollapour

12. Multiple Targeting of HSP Isoforms to Challenge Isoform Specificity and Compensatory Expression
Kisho Ono and Takanori Eguchi

13. Using a Modified Proximity Ligation Protocol to Study the Interaction between Chaperones and Associated Proteins
Simone Baldan, Anatoli B. Meriin, and Michael Y. Sherman

14. Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines
Tanaya Roychowdhury, Anand R. Santhaseela, Sahil Sharma, Palak Panchal, Anna Rodina, and Gabriela Chiosis

15. Molecular Chaperone Receptors: An Update
Thiago J. Borges, Ayesha Murshid, Jimmy Theriault, and Stuart K. Calderwood

16. A Novel Heat Shock Protein 70-Based Vaccine Prepared from DC-Tumor Fusion Cells: An Update
Desheng Weng, Stuart K. Calderwood, and Jianlin Gong

17. Methods to Assess the Impact of Hsp90 Chaperone Function on Extracellular Client MMP2 Activity
Sarah Beth D. Votra, Alexander J. Baker-William, and Dimitra Bourboulia

18. Proteome and Chaperone Analysis of Extracellular Vesicles
Kisho Ono and Takanori Eguchi

19. A Modified Differential Centrifugation Protocol for Isolation and Quantitation of Extracellular Heat Shock Protein 90 (eHsp90)
Cheng Chang, Xin Tang, and Wei Li

20. Immunohistochemistry of Human Hsp60 in Health and Disease: Recent Advances in Immuno-Morphology and Methods for Assessing the Chaperonin in Extracellular Vesicles
Celeste Caruso Bavisotto, Francesco Cappello, Everly Conway de Macario, Alberto J.L. Macario, and Francesca Rappa

21. Multiplex Immunostaining Method to Distinguish HSP Isoforms in Cancer Tissue Specimens
Hotaka Kawai, Kisho Ono, and Takanori Eguchi 

22. Large-Scale Databases and Portals on Cancer Genome to Analyze Chaperone Genes Correlated to Patient Prognosis
Kisho Ono and Takanori Eguchi

23. Immunohistochemical, Flow Cytometric and ELISA-Based Analyses of Intracellular, Membrane-Expressed, and Extracellular Hsp70 as Cancer Biomarkers
Ali Bashiri Dezfouli, Stefan Stangl, Gemma A. Foulds, Philipp Lennartz, Geoffrey J. Pilkington, A. Graham Pockley, and Gabriele Multhoff

Subject Index List…