Cell-Penetrating Peptides: Methods and Protocols

ISBN-13: 9781493961559 / Angielski / Miękka / 2016 / 586 str.

Ulo Langel

Opis

Recently, a new understanding of cell-penetrating peptides has emerged. In this book, expert researchers explore the latest information on cell-penetrating peptides (CPPs). They provide insight into the most important and contemporary areas of CPP research.

Recenzje

From the reviews:

"Prof. Ulo Langel ... rightly explain in a necessary preface that there are in use so many definition for the same things: protein transduction domain (PTDs), Trojan peptides, model amphipathic peptides (MAPs), membrane translocating sequences (MTS) that the best way to refer to all of these molecules is to call all of them cell-penetrating peptides, CPPs. ... Clearly enough, the cell-penetrating peptides volume is surely of great help for basic research and for clinicians engaged in translational medicine." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Spis treści

Cell-penetrating Peptides. Methods and Protocols. Űlo Langel, Editor Table of Contents: Table of Contents Preface Contributors Part I. Introduction 1. Classes and prediction of cell-penetrating peptides Maria Lindgren and Ülo Langel 2. Penetratin story : an overview Edmond Dupont, Alain Prochiantz and Alain Joliot Part II. Methods to test mechanisms of cell-penetrating peptides 3. Testing membrane interactions of CPPs Astrid Gräslund and Lena Mäler 4. Interactions of amphipathic CPPs with model membranes Sébastien Deshayes, Karidia Konate, Gudrun Aldrian, Frédéric Heitz, and Gilles Divita 5. NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems Lena Mäler and Astrid Gräslund 6. Measurements of the intracellular stability of CPPs Ivo R. Ruttekolk, Wouter P. R. Verdurmen, Yi-Da Chung, and Roland Brock 7. Tools for predicting binding and insertion of CPPs into lipid bilayers Paulo Almeida 8. Studies of proteoglycan involvement in CPP-mediated delivery Anders Wittrup, Si-He Zhang and Mattias Belting 9. Uptake Kinetics of cell-penetrating peptides Anders Florén, Imre Mäger, and Ülo Langel 10. Thermodynamics of lipid interactions with cell-penetrating peptides Reto Sauder, Joachim Seelig and André Ziegler 11. Calcium and membrane repair Caroline Palm-Apergi, and Mattias Hällbrink 12. Mapping of protein transduction pathways with fluorescent microscopy Helin Räägel, Pille Säälik, Ülo Langel, and Margus Pooga 13. Insight into cell-entry mechanisms of CPPs by electron microscopy Kärt Padari, Annely Lorents, Eija Jokitalo and Margus Pooga 14. Toxicity methods for CPPs Per Lundin, Samir EL Andaloussi and Ülo Langel 15. Comparison of CPP uptake methods Tina Holm, Samir EL Andaloussi, and Ülo Langel 16. Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery Peter Guterstam, Samir EL Andaloussi and Ülo Langel Part III. Functionality and cell-penetrating peptides 17. Mimicry of protein function with cell-penetrating peptides Henrik Johansson, Samir EL Andaloussi and Ülo Langel 18. Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides Alain Prochiantz 19. Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models Sarah L. Tressel, Georgios Koukos, Boris Tchernychev, Suzanne L. Jacques, Lidija Covic, Athan Kuliopulos 20. Identification and Characterization of Tissue-Specific Protein Transduction Domains using Peptide Phage Display Maliha Zahid, and Paul D. Robbins 21. Applications of Cell Penetrating Peptides as Signal Transduction Modulators for the Selective Induction of Apoptosis Sarah Jones and John Howl Part IV. Applications of CPPs in gene modulation 22. Splice Redirection as a Convenient Assay to Monitor CPP-ON Efficiency and Mechanism Rachida Abes, Andrey A. Arzumanov, Amer F. Saleh, Fatouma Said Hassane, Michael J. Gait and Bernard Lebleu 23. CPP directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy HaiFang Yin, Hong Moulton, Corinne Betts and Matthew Wood 24. PTD-DRBD siRNA delivery Caroline Palm-Apergi, Akiko Eguchi, and Steven F. Dowdy 25. A non-covalent peptide-based strategy for siRNA delivery Laurence Crombez, and Gilles Divita 26. Application of PepFect peptides for the delivery of splice correcting oligonucleotides Samir EL Andaloussi, Taavi Lehto, Per Lundin, and Ülo Langel 27. Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-Nucleoducin Franziska Mussbach, Regina Pietrucha, Buerk Schaefer and Siegmund Reissmann 28. Enhanced cellular delivery of cell-penetrating peptide –peptide nucleic acid conjugates by photochemical internalization (PCI). Takehiko Shiraishi, and Peter E. Nielsen Part V. CPP in vivo and as future drugs 29. Identification of homing peptides using the in vivo phage display technology Antti Rivinoja and Pirjo Laakkonen 30. Measuring the action of CPP-siRNA conjugates in the lung Sterghios A. Moschos, Karen G. Spink, and Mark A. Lindsay 31. Intracellular delivery of nanoparticles with CPPs Rupa Sawant, Vladimir Torchilin 32. Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery Christian Dohmen, Ernst Wagner 33. Cell Penetrating Penta-Peptides (CPP5s) and Bax Inhibiting Peptides (BIPs): Protocol for their application Jose Gomez and Shigemi Matsuyama 34. PAIR technology: exon-specific RNA binding protein isolation in live cells Thomas J. Bell, Emelía Eiríksdóttir, Ülo Langel and James Eberwine 35. Quantitation of cellular and topical uptake of luciferin oligoarginine conjugates Jonathan B. Rothbard and Lisa R. Jones 36. Industrial-scale manufacturing of a possible oligonucleotide cargo CPP based drug Ulf Tedebark, Anthony Scozzari, Oleg Werbitzky, Daniel Capaldi and Lars Holmberg 37. Application of a fusiogenic peptide GALA for intracellular delivery Ikuhiko Nakase, Kentaro Kogure, Hideyoshi Harashima, Shiroh Futaki 38. Therapeutic Applications of Cell-Penetrating Peptides Randolph M. Johnson, Stephen D. Harrison and Derek Maclean 39. Nonclinical and clinical experiences with CPP-based self assembling peptide systems in topical drug development Jacob M. Waugh, Jane Lee, Michael D. Dake and Dan Browne

Blurb

In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.