Cell Membrane Transport: Experimental Approaches and Methodologies

ISBN-13: 9781475796032 / Angielski / Miękka / 2013 / 484 str.

Z. I. Cabantchik; R. Deves; S. Peren

Opis

Experimental science is a complicated creature. At the head there is a Gordian knot of ideas and hypotheses; behind is the accumulated mass of decades of research. Only the laboratory methods, the legs which propel science forward, remain firmly in touch with the ground. Growth, however is uneven; dinosaurs develop by solid means to give a vast body of results, but few ideas. Others sprint briefly to success with brilliant, though ill-supported, ideas. The problems which this book addresses is to maintain an organic unity between new ideas and the current profusion of innovative experimental tools. Only then can we have the framework on which our research thoughts may flourish. The contributors are outstanding scientists in their respective fields and they record here in a clear manner the methodology with which they perform their experiments. They also illustrate some of their most exciting findings. In all chapters the emphasis is on the critical analysis of the methodology which is often avoided in refereed Journals. These techniques are explained in this book in adequate detail. Each chapter is extensively referenced and contains the most recent material available from author's laboratory at the time of going to press.

Spis treści

I: General Methodologies.- 1: Kinetics of Transport: Characterizing the Interaction of Substrates and Inhibitors with Carrier Systems.- 2: Carrier Kinetics Show How the Sodium Pump Uses ATP to Render Pumping of Both Sodium and Potassium Effectively and Suggest a Model for the Action of the F0F1 ATP-ases.- 3: Voltage-Dependent Conductances: Gating Currents and Single-Channel Recordings.- 4: Methods of Determining the Routes of Intestinal Water Transport.- 2: Isotopic Methods for Flux Determination in Various Membrane Preparations.- 5: Characterization of Transport Systems for Solutes at the Blood Side of Endothelial and Parenchymal Cells by Single Circulation Paired Tracer Dilution. A Review of Recent Studies.- 6: Some Inexact Methods for the Analysis of Transport Kinetics Using Dual Tracers.- 7: Transport Studies in Isolated Enterocytes.- 8: Cell Culture in the Study of Epithelial Development and Function.- 9: New Approach to the Study of Transport of Biomolecules by Microcarrier Cell Cultures Perfused in a Column Applying a High Resolution Paired-Tracer Technique.- 10: Methodological Guide for Studying Epithelial Transport with Isolated Membrane Vesicles.- III: Electrophysiological Methods.- 11: The Use of Ion-Selective Microelectrodes to Study Cellular Transport Processes.- 12: Voltage-Dependent Ionic Conductances: “Whole-Cell” Recording by Patch-Clamp Techniques.- 13: Electrophysiological Methods in the Study of Water Transport across Cell Membranes.- IV: Spectroscopic Methods.- 14: A Practical Introduction to the Use of Intracellular Fluorescent Indicators.- 15: Biomedical NMR Spectroscopy and Transport.- 16: Proton NMR Studies of Transmembrane Solute Transport.- 17: Optical and NMR Spectroscopy in the Study of Secretion.- V: Molecular Approaches to Structure and Function of Transporters.- 18: Anion Transport Systems: Continuous Monitoring of Transport by Fluorescence (CMTF) in Cells and Vesicles.- 19: The Molecular Biology of Sugar Transport Proteins.- 20: Chemical and Molecular Probes of Nucleoside Transport Mechanisms in Mammalian Tissues.- VI: Transport Regulation.- 21: Regulation of Junctional Intercellular Communication by Tyrosine-Protein Kinases. Role of the Cellular SRC Gene and Growth Factor Receptors.- 22: Single-Channel and Whole-Cell Patch-Clamp Experiments on Gland Cells: Activation of Ion Channels via Second Messengers.- 23: Application of Rapid Dual Tracer Dilution Techniques for the Study of Endothelial Cell Amino Acid Transport in Perfused Microcarrier Cultures.- Contributors.