Cell-Free Protein Synthesis: Methods and Protocols

ISBN-13: 9781493962549 / Angielski / Miękka / 2016 / 313 str.

Kirill Alexandrov; Wayne A. Johnston

Opis

Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cell-Free Protein Synthesis: Methods and Protocols seeks to serve a wide variety of scientists with its well-honed methodologies.

Spis treści

1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae

Wayne Johnston and Kirill Alexandrov

 

2. Bioinformatics Analysis and Optimization of Cell-Free Protein Expression

Alexander A. Tokmakova, Atsushi Kurotania, Mikako Shirouzu, Yasuo Fukamid, and Shigeyuki Yokoyama

 

3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression

Andrew Kralicek

 

4. One-Pot, Microscale Cell-Free Enzyme Expression and Screening

Aarthi Chandrasekaran and Anup K. Singh

 

5. Cell-Free Translation of Biofuels Enzymes

Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen, and Brian G. Fox

 

6. Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems

Yong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim

 

7. High Level Cell-Free Production of Membrane Proteins with Nanodiscs

Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma, Slawomir Filipek, Xiaoning Wang, Volker Dötsch, and Frank Bernhard

 

8. Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study

Sabrina Guillemer, Cécile Persillon, Jean-Michel Masson, and Gilles Ravot

 

9. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis

Tominari Kobayashi, Kodai Machida, and Hiroaki Imataka

 

10. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates

Kirsten Jackson, Ruba Khnouf, and Z. Hugh Fan

 

11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis

Kiyoshi Ozawa and Ruhu Qi

 

12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis

Kiyoshi Ozawa and Choy Theng Loh

 

13. In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate

Kong-Nan Zhao

 

14. An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA

Xiao Wang, Liang Zhao, and Kong-Nan Zhao

 

15. In vitro Translation Based Protein Kinase Substrate Identification

Szilvia K. Nagy and Tamás Mészáros

 

16. Preparation of Protein Arrays using Cell-Free Protein Expression

Elizabeth A. Cook and Mingyue He

 

17. Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System

Motoaki Wakiyama and Shigeyuki Yokoyama

 

18. Cell-Free Membrane Protein Expression

Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama

 

19. The PURE System for Protein Production

Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda

 

20. A Cell-Free Protein Synthesis System from Insect Cells

Toru Ezure, Takashi Suzuki, and Eiji Ando

 

21. A Cell-Free Expression Platform for Production of Protein Microarrays

Xristo Zárate and David W. Galbraith