Cell-Free Protein Expression

ISBN-13: 9783642639395 / Angielski / Miękka / 2011 / 230 str.

James R. Swartz

Opis

Cell-free protein synthesis is coming of age Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems," edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.

Spis treści

Nemetz Generation of Linear Expression Elements by PCR Nemetz et al. Rapid Protein Engineering by Expression-PCR Stiege Expression-PCR: From Gene Pools to Purified Proteins within One Day Watzele High-throughput expression PCR Used to Systematically Investigate Regulation of Translation Initiation in an E. coli Cell-Free Expression System Gräntzdörffer Reduction of Primer-Dimer Formation During Generation of Expression Fragments by PCR Pelton Isotope Labeling of Proteins for NMR Spectroscopy Using Cell-Free Methods Fernholz Production of a Specifically Labeled Protein in mg Quantities for NMR Analysis Schlößmann In Situ Mono-Biotinylation of Cell-Free Expressed Proteins Using the AviTag Technology Schräml Rapid Generation of Protein Variants and Subsequent Analysis by Surface Plasmon Resonance Bernhard Incorporation of Fluorescence Labels into Cell-Free Produced Proteins Budisa Expression of 'tailor-made' proteins via Incorporation of Synthetic Amino Acids by Using Cell-Free Protein Synthesis Beernink Application of Cell-Free Expression Systems to Proteomic Studies Busso/Kim Screening and Increasing Soluble Expression of Recombinant Proteins using the RTS 100 HY in a 96-Well format Yamane In vitro expression of proteins with disulfide bridges and ist application for high-throughput screening system Kim Cell-free expression of proteins containing multiple disulfide bonds Maurer Cell-free Synthesis of Membrane Proteins on a Preparative Scale Betton Using Maltose-Binding Protein Fragment Complementation to Probe Protein-Protein Interactions by Co-Expression in the RTS System Lorenz In-vitro translation of KRAB Zinc Finger Transcriptional Repressor Proteins and their Interaction withtheir TIF1ß Co-Repressor Lehmann Optimization of Cell-Free Expression of FAD-dependent D-Amino Acid Oxidase Liu Expression of Recombinant Chemokine-like Factor 1 with a Cell-Free Protein Biosynthesis System Bosserhoff Recombinant Expression of Functional Active MIA Protein for Mutation Analysis Using RTS System Bochtler-Hock Cell-Free Expression of the Heterodimeric Protein Penicillin G Amidase in a Functional Active Form Bittorf Cell-Free Expression of the His-Tagged Recombinant Prolactin-like Placenta Protein E Using the RTS 500 System Iskakov Complementary Interaction Between the Central Domain of 18S rRNA and the 5´Untranslated Region of mRNA Enhances Translation Efficiency in Plants Kubick In vitro translation in an insect-based cell-free system