Cell Culture in Pharmaceutical Research

ISBN-13: 9783662030134 / Angielski / Miękka / 2012 / 300 str.

N. E. Fusenig; H. Graf

Opis

Cell biology has made an appreciable impact on the evaluation of physiological and pathophysiological processes leading to a more detailed understanding of the signaling mechanisms by which cells communicate in vivo and in vitro and modify adaptively. By using cell culture models in addition to animal experiments we are now able to better define the overall and the selective potential of drugs. This book is designed to give information on the advantages and limitations and on new aspects and the meaning of cell culture models in pharmaceutical research.

Spis treści

1 Cell Culture Models: Reliable Tools in Pharmacotoxicology?.- 2 Differentiation of Human Monocytes In Vitro: A Model of Macrophage Ontogeny.- 3 Embryonic Stem Cell Derived Cardiogenesis and Myogenesis.- 4 Embryonic Stem Cells as a Tool for Analyzing Mammalian Hematopoietic Development and Disease.- 5 Tight Junctions and the Blood-Brain Barrier.- 6 Carcinogenesis Studies of Human Cells: Reliable In Vitro Models.- 7 The Use of Animal Tumor Models to Study the Role of the Tumor Microenvironment and Paracrine and Autocrine Growth Mechanisms in Metastasis to Specific Sites.- 8 Cellular Models for Angiogenesis Research.- 9 Effects of Progesterone and Antiprogestins on Gonadotropin Secretion in the Model of Cultured Rat Pituitary Cells.- 10 Effect of Progesterone on Proteins Vectorially Secreted by Glandular Epithelial Cells of Guinea Pig Endometrium: Modulation by Homologous Stroma.- 11 Steroids as Negative Regulators of Granulosa Cell Proliferation and Differentiation.- 12 Applications of Expression Vectors Containing Bicistronic Transcription Units in Mammalian Cells.- 13 Regulation of Progesterone Receptor Activity in Cell Culture Systems and Cell-Free Transcription.