Cell Culture Engineering: Recombinant Protein Production

ISBN-13: 9783527343348 / Angielski / Twarda / 2019 / 440 str.

Gyun Min Lee; Helene Faustrup Kildegaard; Sang Yup Lee

Opis

Part of the "Advanced Biotechnology" series, this volume covers all important aspects of cell culture engineering as applied to the production of recombinant proteins for therapeutic use, from molecular biology to bioprocess engineering.

Spis treści

1. A platform technology for therapeutic protein production
2. Cell line development for therapeutic protein production
3. Development of a transient gene expression upstream platform
4. CHO genome: Enhancing product and bioprocess attributes using genome–scale models of CHO metabolism
5. Epigenetics and stability: An investigation on genome heterogeneity and stability and on what controls cell behavior
6. CHO synthetic biology
7. Omics data and mammalian systems biotechnology: an integrative framework for combining in silico modeling and multi–omics datasets in different CHO parental cell lines
8. Mammalian gene editing tools
9. Cell Engineering
10. Metabolite Profiling of Mammalian Cells
11. Application of 13C flux analysis for cell engineering
12. Host cell protein impurity
13. Batch and fed–batch culture
14. Perfusion culture
15. Process analytical technology and quality–by–design for animal cell culture
16. Development of a scale down cell culture model using multivariate analysis as a qualification tool

O autorze

Gyun Min Lee is Professor at the Department of Biological Sciences at KAIST, South Korea, since 2001 and heads the Animal Cell Engineering Laboratory. He studied chemical engineering at Seoul National University, South Korea, and received his Ph.D. from the University of Michigan, USA, in 1990. In 1991, he returned to South Korea and held several positions at KAIST until he became full professor in 2001. From 1998 to 1999, Gyun Min Lee was a visiting scientist at the University of California in San Diego, USA. At present, he is a scientific director at the Novo Nordisk Foundation Center for Biosustainability at the Technical University of Denmark. He received several national awards and published more than 200 publications in the field of cell culture and protein production.

Helene Faustrup Kildegaard is a senior researcher and Co–PI for the CHO Cell Line Engineering and Design section at the Novo Nordisk Foundation Center for Biosustainability at the Technical University of Denmark (DTU). In 2009, she received her Ph.D. in molecular and cell biology from DTU, where she held several positions afterwards. From 2003 to 2004, she studied abroad at Michigan Technological University, USA, and was a visiting researcher at the University of Natural Resources and Life Sciences Vienna, Austria, in 2016.