Ccn Proteins: Methods and Protocols

ISBN-13: 9781493981977 / Angielski / Miękka / 2018 / 576 str.

Masaharu Takigawa

Spis treści

The CCN Proteins: An Overview.- Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues .- Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols.- Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.- Western Blotting Analysis of CCN Proteins.-  Immunohistochemical Analysis of CCN Proteins.- Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis.-  Production of Recombinant CCN2 Protein in Escherichia. Coli.-  Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis.- Production Of Recombinant CCN2 Protein By Mammalian Cells.- In Vitro Transfection with and Expression of CCN Family of Genes.- Preparation of Module Specific Antibodies against CCN2.- ELISA of CCN Proteins in Body Fluids Including Serum And Plasma.- Analysis of Signaling Pathways Activated by CCN Proteins.- Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System.- Protocols For Screening Peptide Motifs Binding To CCN Proteins.- Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR).-  Promoter Analysis of CCN Genes.- Analysis Of Posttranscriptional Regulation Of CCN Genes.-  Protein Imaging of CCN2 and CCN3 in Living Cells.-  Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins.-  Protocol for Measuring Angiogenic Activities of CCN Proteins.- Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins.- Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies.- In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins.- Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress.- The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice.- Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters.- Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.- Construction and Analysis of an Allelic Series of CCN1 Knockin Mice.-  Production and Analysis of Conditional KO Mice of CCN2 in Kidney.- Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice.- Analysis of Transcytosis of CCN2 by Chondrocytes.- CCN2 in Skin Fibrosis.- Studying the CCN Proteins in Fibrosis.- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Kidney Fibrosis.- Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Liver Fibrosis.- Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis.- TGF-Β1 snd CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.-  CCN Detection of Cancer Tissues by Immunohistochemistry Staining.- Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique.- Analysis of Pathological Activities of CCN Proteins in Bone Metastasis.- Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy.-  Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System.- An Analysis of Pathological Activities of CCN Proteins in Joint Disorders−Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells−.-  Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies.