Caspases, Paracaspases, and Metacaspases: Methods and Protocols

ISBN-13: 9781493903566 / Angielski / Twarda / 2014 / 266 str.

Peter Bozhkov; Guy Salvesen

Opis

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals. Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death. The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.

Spis treści

Part I: Caspases

1. General in vitro Caspase Assay Procedures

Dave Boucher, Catherine Duclos, Jean-Bernard Denault

 

2. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity

Marcin Poręba, Aleksandra Stróżyk, Paulina Kasperkiewicz, Marcin Drąg

 

3. Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform)

Melissa M. Dix, Gabriel M. Simon, Benjamin F. Cravatt

 4. Caspase-2 Protocols

Loretta Dorstyn and Sharad Kumar

 

5. Caspase-14 Protocols

Mami Yamamoto-Tanaka and Toshihiko Hibino

 

6. Caspase Protocols in Caenorhabditis elegans

Eui Seung Lee & Ding Xue

 

7. Detecting Caspase Activity in Drosophila Larval Imaginal Discs

Caitlin E. Fogarty and Andreas Bergmann

 

8. Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract

Francis McCoy, Rashid Darbandi, and Leta K. Nutt

 

9. Caspase Protocols in Mice

Varsha Kaushal, Christian Herzog, Randy S. Haun, and Gur P. Kaushal

 

10. Measurement of Caspase Activation in Mammalian Cell Cultures

Magnus Olsson and Boris Zhivotovsky

 

 

Part II: Paracaspases and Metacaspases

11. Detection and Measurement of Paracaspase MALT1 Activity

Stephan Hailfinger, Christiane Pelzer, and Margot Thome

 

12. Leishmania Metacaspase: an Arginine-Specific Peptidase

Ricardo Martin, Iveth Gonzalez and Nicolas Fasel

 

13. Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases
Karen McLuskey, Catherine X. Moss, and Jeremy C. Mottram

 

14. Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1

Amit Shrestha, Robin E. C. Lee, and Lynn A. Megeney

 

15. Plant Metacaspase Activation and Activity

Elena A. Minina, Simon Stael, Frank Van Breusegem, and Peter V. Bozhkov

 

16. Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC

Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, and Kris Gevaert