Cartilage and Osteoarthritis

ISBN-13: 9781588295057 / Angielski / Twarda / 2004 / 346 str.

Frederic de Ceuninck; Philippe Pastoureau; Massimo Sabatini

Opis

An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

Recenzje

"This is a high quality laboratory manual that provides detailed instructions for conducting experiments directed at studying the role of cartilage in osteoarthritis. Overall, this would be a worthwhile book to keep in the laboratory for anyone studying the molecular mechanisms of connective tissue diseases."-Doody's Health Sciences Book Review Journal

Spis treści

Generation and Use of Transgenic Mice As Models of Osteoarthritis Anna-Marja Säämänen and Eero Vuorio Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick Garnero Histological and Immunohistological Studies on Cartilage James Melrose, Susan Smith, and Peter Ghosh Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe James Melrose and Susan Smith Methods for Cartilage and Subchondral Bone Histomorphometry Philippe Pastoureau and Agnès Chomel Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritis Martin Judex, Elena Neumann, Steffen Gay, and Ulf Müller-Ladner Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopy Stephan Soeder, Alexander Kuhlmann, and Thomas Aigner Molecular and Biochemical Assays of Cartilage Components Caroline D. Hoemann Mechanical Characterization of Native and Tissue-Engineered Cartilage Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. Sah Noninvasive Study of Human Cartilage Structure by MRI Felix Eckstein High-Resolution MRI Techniques for Studies in Small-Animal Models Olivier Beuf High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography Lydia Wachsmuth and Klaus Engelke High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes Amena Saïed and Pascal Laugier Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Jürgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Jörn Kaufmann, and Klaus Arnold Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseases Jürgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus Arnold Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods Daniel Huster, Jürgen Schiller, and Klaus Arnold Computerized Protocol for Anatomical and Functional Studies of Joints Sandra Martelli and Stefano Zaffagnini Index

Blurb

Osteoarthritis (OA), the most common form of arthritis, imposes a significant burden of suffering on a growing population of the elderly. Even today, its poorly understood pathophysiology limits the discovery of molecular targets for pharmacological intervention and there are few effective medical treatments beyond pain control and surgery. In Cartilage and Osteoarthritis a distinguished panel of researchers, physicians, and opinion leaders in this challenging field describe their updated classical, but still evolving, techniques, as well as many emerging methods that promise to add significantly to our understanding of cartilage metabolism in health and disease. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for studies-at the tissue level-of turnover, structure, and functioning in normal and diseased cartilage by invasive and noninvasive means. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Volume 1: Cellular and Molecular Tools, describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells.
Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists alike with an indispensable collection of readily reproducible protocols for new experiments-from the cellular to the animal level-designed to more clearly describe the pathophysiology of cartilage, as well as to discover novel molecular targets for pharmacological intervention.