Capillary Electrophoresis of Nucleic Acids

ISBN-13: 9781617371868 / Angielski / Miękka / 2010 / 484 str.

Keith R. Mitchelson; Jing Cheng

Opis

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Recenzje

"These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)...Chapters on the use of microchip and mass spectrometric detection are also included...Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE." - Clinical Chemistry

Review for both volumes:

"This two-volume set of books comprises 64 chapters of protocols for the analysis of just about every form and size of nucleic acids that one can imagine. Thus it rates near an "A" for its comprehensiveness." -Analytical Chemistry

"...rates near an "A" for its comprehensiveness." - Analytical Chemistry

Spis treści

Part I. Analytical Considerations for Capillary Electrophoresis. Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis, Keith R. Mitchelson. DNA Separation Mechanisms During Capillary Electrophoresis, Gary W. Slater, Claude Desruisseaux, and Sylvain J. Hubert. Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis, Joseph M. Devaney and Michael A. Marino. Quantitative Measurements, Bruce McCord. Microchip-Based Capillary Electrophoresis Systems, Wenzhang Xie, Rong Yang, Junquan Xu, Liang Zhang, Wanli Xing, and Jing Cheng. DNA Sequencing by Capillary Array Electrophoresis, Norman J. Dovichi and JianZhong Zhang. Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis, John Bashkin. Part II. Capillary Matrix: Considerations for Capillary Electrophoresis. Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA, Christoph Heller. Capillary Coatings: Choices for Capillary Electrophoresis of DNA, Marcella Chiari and Marina Cretich. Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis, Mark A. Quesada and Steve Menchen. Sieving Matrix Selection, Tim Wehr, Mingde Zhu, and David T. Mao. Parameters Affecting Capillary Electrophoretic Separation of DNA, Haleem J. Issaq. Part III. Electrophoresis Matrix: Applications for DNA Fragment Sizing. DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals, Yingjie Liu and Randolph L. Rill. Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material, Yongseong Kim and Edward S. Yeung. DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium, Benjamin Chu, Tianbo Liu, Chunhung Wu, and Dehai Liang. DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries, Karel Klepárník, Zdenka Malá, and Petr Bocek. Capillary Electrophoresis with Glycerol as anAdditive, Keith R. Mitchelson and Jing Cheng. Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels, Anders K. Palm. Part IV. Practical Applications for Large DNA Fragment Sizing and Fragment Collection. Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments, Christoph Heller, Soffia Magnúsdóttir, and Jean-Louis Viovy. Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments, Michael D. Morris, Jeffrey J. Schwinefus and Olivia de Carmejane. Collection of Capillary Electrophoresis Fractions on a Moving Membrane, Soffia Magnúsdóttir, Christoph Heller, Phillipe Sergot, and Jean-Louis Viovy. Separation of Supercoiled DNA Using Capillary Electrophoresis, David T. Mao and Roy M. A. Lautamo. Part V. Practical Applications for Oligonucleotide and Nucleotide Analysis. Quality Control of Nucleotides and Primers for PCR, Martin J. Pearce and Nigel D. Watson. Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis, Lawrence A. DeDionisio. Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides, G. Susan Srivatsa, Roya Pourmand, and Steven Winters. Capillary Electrophoresis Separation of Ribonucleosides, Dan-Ke Xu and Hong-Yuan Chen. On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries, Iris Barmé and Gerard J. M. Bruin. Part VI. Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA. Capillary Electrophoresis: Mass Spectrometric Analysis of DNA Adducts, Lisa A. Marzilli, Cheryl Koertje, and Paul Vouros. Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis, Zeena E. Nackerdien, Barbara A. Siles, Stacey A. Nevins, and Donald H. Atha. Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis, James Z. Xing, Trevor Carnelley, Jane Lee, William P. Watson, Ewan Booth, Michael Weinfeld, and X. Chris Le. Analysis

Blurb

The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has generated a host of DNA analytical procedures. In these novel and wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This first volume of the set, Introduction to the Capillary Electrophoresis of Nucleic Acids, covers the practical and theoretical considerations behind the use of capillary electrophoresis for the analysis of small oligonucleotides and modified nucleotides. Along with detailed instructions ensuring ready reproducibility, these protocols offer time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE. Several chapters are devoted to the analysis of small therapeutic oligonucleotides, nucleosides, and ribonucleotides by CE. The companion volume, Practical Applications of Capillary Electrophoresis, addresses techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.