Capillary Electrophoresis of Biomolecules: Methods and Protocols

ISBN-13: 9781627032957 / Angielski / Twarda / 2013 / 358 str.

Nicola Volpi; Francesca Maccari

Opis

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.

Spis treści

1. Recent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery

Chenchen Wang, Xueping Fang, and Cheng S. Lee

2. Capillary Electrophoresis-mass Spectrometry of Carbohydrates

^{Joseph Zaia}

^{3. Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis}

^{M.C. Breadmore}

^{4. Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis}

^{Mitsuru Abo, Li-ping He, Kae Sato and Akira Okubo}

^{5. Determination of Mono-Di-and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contractless Conductivity Detection}

^{Claudimir Lucio do Lago, Thiago Nogueira, Lucas Blanes, and Renata Mayumi Saito}

^{6. Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis}

^{Toshiaki HATTORI, Nobuhiro ANRAKU and Ryo KATO}

^{7. Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides}

^{Yuqing Chang, Bo Yang, Amanda Weyers, Robert J. Linhardt}

 ^{8. High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes}

^{Mehdi Moini}

9. Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow

Ming-Mu Hsieh, Po-Ling Chang

 10. Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection

Angelo Zinellu, Salvatore Sotgia, Luca Deiana,  Ciriaco Carru

11. Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies

Carolina Simo, Alejandro Cifuentes, Václav Kašička

 12. High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS

Amaya Albalat, Vasiliki Bitsika, Petra Zurbig, Justyna Siwy, and William Mullen

13. Contribution of CE to the Analysis of Protein or Peptide Biomarkers

Kiarach Mesbah, Romain Verpillot, François de l’Escaille, Jean Bernard Falmagne, Myriam Taverna

 14. Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis

^{R. Nehmé and C. Perrin}

^{15. Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids}

^{Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa, Mercedes de Frutos}

 ^{16. Automated Capillary Electrophoresis in the Screening
for Haemoglobinopathies}

^{Frédéric Cotton, Fleur Wolff, Béatrice Gulbis}

17. Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection

Cristina Pelaez-Lorenzo, Maria Teresa Veledo, Ramon Gonzalez, Mercedes de Frutos, Jose Carlos Diez-Masa

 18. Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits

Tomoyuki Katsube-Tanaka

19. Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis

Benjamin J. Alper and Walter K. Schmidt

 20. Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei

Julie M. Chandler, Heather R. Trenary, Gary R. Walker, and, Jr.

 21. Capillary Electrophoretic Enzyme Assays

Gerhard K. E. Scriba, Hans Abromeit, Martina Hense and Yi Fan

22. A Methodology for Detection and Quantification of Esterase Activity

Ana L. Simplício, Ana S. Coroadinha, John F. Gilmer and Joana Lamego

 23. Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique

Chao Liu, Qianqian Zhang, Jingwu Kang

 24.  Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis

Philip M. Yangyuoru, Latanya Hammonds-Odie, Simon M. Mwongela

25. Assessment of DNA damage by Micellar Electrokinetic Chromathography

Paolo Fattorini, Giorgio Marrubini, Pierangela Grignani, Solange Sorçaburu-Cigliero, Carlo Previdere`