Calcium-Binding Proteins and Rage: From Structural Basics to Clinical Applications

ISBN-13: 9781627032292 / Angielski / Twarda / 2013 / 424 str.

Claus Heizmann

Opis

A major direction in medical research leading to clinical applications targets the regulation of intracellular calcium and the various human diseases associated with an altered homeostasis of this global second messenger. These diseases include, for example: cardiomyopathy, inflammation, brain disorders, diabetes and cancer. In Calcium-Binding Proteins and RAGE: from Structural Basics to Clinical Applications, expert researchers in the field detail many of the methods which are now commonly used to study calcium binding proteins. These methods and techniques, such as calcium-measurements, screening methods, clinical chemistry, and therapy, are generally applicable to many other areas of basic and medical research as well as to diagnostics. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Calcium-Binding Proteins and RAGE: from Structural Basics to Clinical Applications underlines the diagnostic and clinical importance of this family of proteins in human diseases and as drug targets.

Spis treści

Part I: Intracellular Ca²⁺- Measurements and Cation Interactions with EF—Hand Proteins

1. Measurement of Intracellular Ca²⁺ Concentration in Single Cells using Ratiometric Calcium Dyes

Sonal Srikanth and Yousang Gwack

 

2.  Divers Models of Divalent Cation Interaction to Calcium-binding Proteins: Techniques and Anthology

Jos A. Cox

 

3. Probing Ca²⁺ Binding Capability of Viral Proteins with the EF-hand Motif by Grafting Approach

Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang

 

Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

4.  New Aspects of Calmodulin-calmodulin Binding Domains Recognition.

Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech

 

5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells

Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann

 

6.  X-ray Structural Analysis of S100 Proteins

Dr. Günter Fritz

 

7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies

Hao Huang and Hans J. Vogel

 

8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties

Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña

 

 

9. Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization

Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji

 

10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter

 

Part III: Interaction of Ca²⁺-Binding Proteins with Targets and Receptors/RAGE

 

11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin

 

12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners

Kanae Osugi, Hideki Shibata and Masatoshi Maki

 

13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance

Estelle Leclerc

 

14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

Stefan W. Vetter

 

Part IV: RAGE-Meditated CELL Signalling and Pathology

15. RAGE-mediated Cell Signalling

Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala,

 

16. RAGE Splicing Variants in Mammals

Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar

 

 

Part V: Ca²⁺-Binding Proteins in Human Diseases and As Drug Targets

17.  Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example

Joerg Heineke, MD

 

18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy

Danna B. Zimmer, Rena G. Lapidus  and David J. Weber

 

19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness

Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

4.  New Aspects of Calmodulin-calmodulin Binding Domains Recognition.

Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech

 

5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells

Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann

 

6.  X-ray Structural Analysis of S100 Proteins

Dr. Günter Fritz

 

7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies

Hao Huang and Hans J. Vogel

 

8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties

Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña

 

 

9. Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization

Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji

 

10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter

 

Part III: Interaction of Ca²⁺-Binding Proteins with Targets and Receptors/RAGE

 

11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin

 

12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners

Kanae Osugi, Hideki Shibata and Masatoshi Maki

 

13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance

Estelle Leclerc

 

14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

Stefan W. Vetter

 

Part IV: RAGE-Meditated CELL Signalling and Pathology

15. RAGE-mediated Cell Signalling

Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala,

 

16. RAGE Splicing Variants in Mammals

Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar

 

 

Part V: Ca²⁺-Binding Proteins in Human Diseases and As Drug Targets

17.  Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example

Joerg Heineke, MD

 

18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy

Danna B. Zimmer, Rena G. Lapidus  and David J. Weber

 

19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness

Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

Sonal Srikanth and Yousang Gwack

 

2.  Divers Models of Divalent Cation Interaction to Calcium-binding Proteins: Techniques and Anthology

Jos A. Cox

 

3. Probing Ca²⁺ Binding Capability of Viral Proteins with the EF-hand Motif by Grafting Approach

Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang

 

Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

4.  New Aspects of Calmodulin-calmodulin Binding Domains Recognition.

Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech

 

5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells

Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann

 

6.  X-ray Structural Analysis of S100 Proteins

Dr. Günter Fritz

 

7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies

Hao Huang and Hans J. Vogel

 

8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties

Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña

 

 

9. Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization

Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji

 

10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter

 

Part III: Interaction of Ca²⁺-Binding Proteins with Targets and Receptors/RAGE

 

11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin

 

12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners

Kanae Osugi, Hideki Shibata and Masatoshi Maki

 

13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance

Estelle Leclerc

 

14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

Stefan W. Vetter

 

Part IV: RAGE-Meditated CELL Signalling and Pathology

15. RAGE-mediated Cell Signalling

Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala,

 

16. RAGE Splicing Variants in Mammals

Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar

 

 

Part V: Ca²⁺-Binding Proteins in Human Diseases and As Drug Targets

17.  Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example

Joerg Heineke, MD

 

18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy

Danna B. Zimmer, Rena G. Lapidus  and David J. Weber

 

19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness

Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

Part II: Expression, Purification, Biochemical, and Structural Analyses and Cellular Localization

4.  New Aspects of Calmodulin-calmodulin Binding Domains Recognition.

Emilie Audran, Rania Dagher, Sophie Gioria, Marie-Claude Kilhoffer and Jacques Haiech

 

5. Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells

Kenji Kizawa, Masaki Unno, Hidenari Takahara, Claus W. Heizmann

 

6.  X-ray Structural Analysis of S100 Proteins

Dr. Günter Fritz

 

7. Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies

Hao Huang and Hans J. Vogel

 

8. Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties

Nuria Parody, Miguel Ángel Fuertes, Carlos Alonso, Yago Pico de Coaña

 

 

9. Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization

Anisuzzaman, M. Khyrul Islam, M. Abdul Alim and Naotoshi Tsuji

 

10. Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin

Johannes Hradsky, Marina Mikhaylova, Anna Karpova, Michael R. Kreutz, and Werner Zuschratter

 

Part III: Interaction of Ca²⁺-Binding Proteins with Targets and Receptors/RAGE

 

11. NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides

Steven M. Damo, Michael D. Feldkamp, Benjamin Chagot, Walter J. Chazin

 

12. Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners

Kanae Osugi, Hideki Shibata and Masatoshi Maki

 

13. Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance

Estelle Leclerc

 

14. Phage Display Selection of Peptides that Target Calcium-Binding Proteins

Stefan W. Vetter

 

Part IV: RAGE-Meditated CELL Signalling and Pathology

15. RAGE-mediated Cell Signalling

Ari Rouhiainen, Juha Kuja-Panula, Sarka Tumova, Heikki Rauvala,

 

16. RAGE Splicing Variants in Mammals

Katharina Anna Sterenczak, Ingo Nolte, and Hugo Murua Escobar

 

 

Part V: Ca²⁺-Binding Proteins in Human Diseases and As Drug Targets

17.  Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example

Joerg Heineke, MD

 

18. In Vivo Screening of S100B Inhibitors for Melanoma Therapy

Danna B. Zimmer, Rena G. Lapidus  and David J. Weber

 

19. Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness

Kadri Vaidla, Janne Üksti, Christina Zeitz, Eneli Oitmaa

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

 

20. Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders

Hiroyuki Shimizu and Masatomo Mori

 

 

 Part VI: Cell Line Authentication

21. Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines

Robert S. McLaren, Yvonne Reid, and Douglas R. Storts

 

 

Part VII: Biomarkers, Diagnostic, Clinical Chemistry, and Gene Therapy

22. Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS

Massimo Castagnola, Tiziana Cabras, Federica Iavarone, Chiara Fanali and Irene Messana

 

23. Clinical use of the Calcium-Binding S100B Protein

Ramona Astrand, Johan Undén, and Bertil Romner

 

24. Sensible use of High-sensitivity Troponin Assays

Danielle Hof , Roland Klingenberg, and Arnold von Eckardstein

 

25. S100A1 Gene Therapy in Small and Large Animals

Patrick Most, Philip Raake, Christophe Weber, Hugo A. Katus and Sven T. Pleger

Blurb

A major direction in medical research leading to clinical applications  targets the regulation of intracellular calcium and the various human diseases associated with an altered homeostasis of this global second messenger. These diseases include, for example: cardiomyopathy, inflammation, brain disorders, diabetes and cancer. In Calcium Binding Proteins and RAGE: from Structural Basics to Clinical Applications, expert researchers in the field detail many of the methods which are now commonly used to study calcium binding proteins. These methods and techniques, such as calcium-measurements, screening methods, clinical chemistry, and therapy, are generally applicable to many other areas of basic and medical research as well as to diagnostics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Calcium Binding Proteins and RAGE: from Structural Basics to Clinical Applications underlines the diagnostic and clinical importance of this family of proteins in human diseases and as drug targets.