Calcium Binding Proteins

ISBN-13: 9780470525845 / Angielski / Twarda / 2011 / 592 str.

Eugene Permyakov; Robert H. Kretsinger; E. A. Perm'iakov

Opis

Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry, and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.

Spis treści

Preface.

1. Historical Perspective.

2. Physiological Processes Involving Calcium Binding Proteins.

3. Comparison of the Ca²⁺ Ion with Other Metal Cations.

4. Complexes of Calcium and Other Cations with Low Molecular Weight.

5. Stoichiometry, Kinetics, and Thermodynamics of Calcium Binding.

6. Experimental Methods Used to Study Calcium Binding to Proteins.

7. Structure and Evolution of Proteins.

8. Protein Complexes with Metals Other than Calcium.

9. Nonessential Metals.

10. Parvalbumin.

11. EF–Hand Proteins.

12. Cytosolic Calcium Binding Proteins Lacking EF–Hands.

13. Extracellular Calcium Binding Proteins.

14. Interactions of Calcium Binding Proteins with Other Metal Ions.

15. Interactions of Calcium Binding Proteins with Other Proteins and with Membranes.

16. Genetic Engineering of Calcium Binding Proteins.

References.

Index. 

O autorze

Eugene A. Permyakov received his PhD in physics and mathematics at the Moscow Institute of Physics and Technology in 1976 and defended his Doctor of Science dissertation in biology at Moscow State University in 1989. From 1970 to 1994, he worked at the Institute of Theoretical and Experimental Bio–physics of the Russian Academy of Sciences. Since 1994 he has been the Director of the Institute for Biological Instrumentation of the Russian Academy of Sciences. He is known for his work on metal binding proteins and the intrinsic fluorescence method. His primary research focus is the study of physico–chemical and functional properties of metal binding (especially calcium–binding) proteins.

Robert H. Kretsinger received his PhD in biophysics at the Massachusetts Institute of Technology under the supervision of Alex Rich in 1964. In 1967 he set up a protein crystallography lab in the Department of Biology at the University of Virginia, where he¿presently teaches, and determined the crystal structure of parvalbumin as well as describing the EF–hand domain in 1971. He has subsequently investigated the structures, functions, and evolution of various EF–hand homologs, along with other protein families. From 1978 to 1995, he directed a national facility to build multiwire area detectors for x–ray diffraction and to collect data from protein crystals.

Blurb

An up–to–date exploration on the latest developments in all the major families of calcium binding proteins

Playing a prominent role as a signal transducer, calcium is an important participant in the communication pipeline directing cellular activity. This expert guide explains the unique and highly diverse functions of calcium in biology, culminating with a discussion of the calcium binding proteins that act as secondary messengers in cells and are responsible in controlling vital bodily mechanisms such as muscle contraction and the regulation of enzymes.

A comprehensive volume reflecting breakthrough science, Calcium Binding Proteins:

• Contains current information on all major families of calcium binding proteins including their structural, physico–chemical and functional properties, as well as their evolution

• Discusses techniques that underlie the description of proteins, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry, and crystallography

• Describes the structures, physical characteristics, functions, and general patterns in the evolution of calcium binding proteins

This book relies on the most current biological findings to cover the patterns of biochemical phenomena such as calcium homeostasis, min–eralization, and cell signaling involving specific proteins. Summarizing ongoing studies and presenting general hypotheses that help to focus future research, Calcium Binding Proteins provides newcomers to this field as well as established professionals with a conceptual framework instru–mental in advancing their scientific pursuits.