C. Elegans: Methods and Applications

ISBN-13: 9781588295972 / Angielski / Twarda / 2006 / 292 str.

Kevin Strange

Opis

Molecular biology has driven a powerful reductionist, or molecule-c- tric, approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of naive reductionism. Red- tionist methods will continue to be an essential element of all biological research efforts, but naive reductionism, the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2 5). The systems of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or emergent properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e."

Spis treści

An Overview of C. elegans Biology Kevin Strange Comparative Genomics in C. elegans, C. briggsae and Other Caenorhabditis Species Avril Coghlan, Jason E. Stajich and Todd W. Harris WormBase: Methods for Data Mining and Comparative Genomics Todd W. Harris and Lincoln D. Stein C. elegans Deletion Mutant Screening Robert J. Barstead and Donald G. Moerman Insertional Mutagenesis in Caenorhabditis elegans Using the Drosophila Transposon Mos1: A Method for the Rapid Identification of Mutated Genes Jean-Louis Bessereau SNP Mapping M. Wayne Davis and Marc Hammarlund Creation of Transgenic Lines Using Microparticle Bombardment Methods Vida Praitis Construction of Plasmids for RNAi and In Vitro Transcription of dsRNA Lisa Timmons Delivery Methods for RNAi in Caenorhabditis elegans Lisa Timmons Functional Genomic Approaches in C. elegans Todd Lamitina Assays for Toxicity Studies in C. elegans with Bt Crystal Proteins Larry J. Bischof, Danielle L. Huffman and Raffi V. Aroian Fluorescent Reporter Methods Harald Hutter Electrophysiological Analysis of Neuronal and Muscle Function in C. elegans Michael M. Francis and Andres Villu Maricq Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis Michael A. Miller Preservation of C. elegans Tissue via High-Pressure Freezing and Freeze-Substitution for Ultrastructural Analysis and Immuno-Cytochemistry Robby M. Weimer Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants Keith Nehrke Automated Imaging of C. elegans Behavior Christopher J. Cronin, Zhaoyang Feng and William R. Schafer Intracellular Ca2+ Imaging in Caehorhabditis elegans Rex A. Kerr and William R. Schafer In Vitro Culture of C. elegans Somatic Cells Kevin Strange and Rebecca Morrison Techniques for Analysis, Sorting, and Dispensing of C. elegans on the COPAS Flow Sorting System Rock Pulak et al.